Document Type : Original Article
Authors
1
Department Plant Biochemistry, Agriculture and Biology Division,National Research Center
2
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Cairo, Egypt.
3
Biostatistics Unit, Theodor Bilharz Research Institute,
4
Parasitology and animal disease department
5
Parasitology and Animal Diseases Department, Veterinary Research Division, National Research Centre
6
National Research Centre, Dokki, Giza, Egypt.
Abstract
The larvicidal activities of Ocimumbasilicum (O. basilicum), Citrus limon (C.limon), Syzygiumaromaticum (S. aromaticum), and Piper nigrum (P. nigrum) extracts were evaluated against 3rd instar Cephalopinatitillator larvaeas an alternative to chemical drugs. In addition, the antioxidant capacity of these crude extracts was measured by four common methods. The P. nigrum seed extract possessed the highest antioxidant activity, highest total phenolic and flavonoid contents among all the investigated four plant extracts. The DPPH (1, 1-Diphenyl-2-Picrylhydrazyl) radical scavenging activity was 73.73±0.14% and the reducing power activity was 2.01±0.007). Ferric reducing power ability (FRAP) was 5327μM Trolox /100 g DW, while ABTS radical scavenging ability (2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) found to be 75.91±0.59% at the concentration (50 µg/ ml). According to the mortality percentages of C. titilator larvae and the LC50, 90 of S.aromaticum extract were found to be more effective with an increase in dose followed by C.limon, O.basilicum, and P. nigrum. Also, by using light and scanning electron microscope (SEM) the morphological changes that occurred 24 hr at 1% of C.limon extract were filmed and the examined larva was exhibited extensive swelling of the integument. Also, posterior spiracles were showed severe damage and shrinkage of the internal structure. In conclusion, all the investigated plant extracts exhibited good antioxidant activity. The current study offers an opportunity for new compounds, which is a cheap alternative for the more costly larvicides.
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