Resveratrol inhibits Cell Cycle Dynamics, Caspase Activation, and Programmed Cell Death: Implications for Cancer Treatment in MCF-7 Cells

Hamad, Sura H.; Musleh, Zinah Hashim; Nasir, Kawther M.; Hameed, Ashwaq T; Eskander, Georgette

doi:10.21608/ejvs.2024.263893.1787

Resveratrol inhibits Cell Cycle Dynamics, Caspase Activation, and Programmed Cell Death: Implications for Cancer Treatment in MCF-7 Cells"

Document Type : Original Article

Authors

¹ Al Maaref University College. Iraq

² Ministry of Education, General Directorate of Education, Anbar

³ College of Education for women, University of Anbar, Iraq

⁴ University of Anbar, College of Education for women

⁵ Faculty of Pharmacy, Ain Shams University, Postgraduate Student, Cairo, Egypt

10.21608/ejvs.2024.263893.1787

Abstract

Resveratrol is a plant-derived polyphenol known for its anti-inflammatory and anti-tumor properties in laboratory models and living organisms. Recent studies suggest that certain analogs of resveratrol may exhibit more effective anti-tumor factors. This study investigates the impact of resveratrol on the cell cycle of MCF-7 cells at concentrations of 1.5 and 2µg\ ml. Resveratrol, at 2µg\ml, significantly alters the cell cycle distribution, indicating a concentration-dependent effect. Doxorubicin serves as a positive control and induces G1 phase arrest. These results contribute to understanding the potential mechanisms of resveratrol in cancer treatment and shed light on its impact on cell cycle dynamics. Additionally, the study explores the effects of different concentrations of resveratrol on the activity of Caspase 8, using doxorubicin as a positive control for comparison. Human cell lines were exposed to varying concentrations of resveratrol (1.5 µg\ ml and 2 micrograms/ml), and Caspase 8 activities were compared with both untreated cells (control group) and those treated with doxorubicin. The average Caspase 8 activity and standard deviation were calculated for each treatment group, and the statistical significance was determined. this study highlights the concentration-dependent effects of resveratrol on the cell cycle distribution of MCF-7 cells, suggesting its potential as a therapeutic agent in cancer treatment. Furthermore, the investigation of Caspase 8 activity provides additional insight into the anti-cancer properties of resveratrol in breast cells.

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