Molecular Detection and Characterization of Haemoplasmas in Different Animal Species in Egypt

Eissa, Sabry Ismail; Abdelaziz, Eid El Saeed; Hassan, Abdelwahed Mahmuod; Mohamed, Yousreya Hashim; Ouda Ahmed, Sahar El-Sayed; Elshabiny, Laila Mostafa

doi:10.21608/ejvs.2023.245264.1658

Molecular Detection and Characterization of Haemoplasmas in Different Animal Species in Egypt

Document Type : Original Article

Authors

¹ Mycoplasma department AHRI,ARC,GIZA,Egypt

² Mycoplasma Department ,AHRI,ARC,GIZA,EGYPT.

³ Mycoplasma Department AHRI,ARC,GIZA,EGYPT.

⁴ Mycoplasma Department AHRI,ARC,GIZA.EGYPT

⁵ Department of mycoplasma AHRI, ARC, Giza Egypt

⁶ Mycoplasma Department AHRI,ARC,Giza,EGYPT

10.21608/ejvs.2023.245264.1658

Abstract

Background:
Hemotropic mycoplasmas (HM) the non-cultivable bacteria cause infectious anemia in many animals and humans.
Objective: Molecular detection, partial sequencing and phylogenetic analysis of the 16S rRNA of haemoplasmas in some animal species in Egypt.
Method: Blood samples with anticoagulant were collected from jugular vein of camels, sheep, goats, dogs and cats. DNA was extracted and different PCR assays were applied and sequenced.
Results: Candidatus M. haemoovis was detected in 10% of sheep in Beni- Swef governorate and in 14.46% of goats in Gharbia and the PCR resulted in the specific fragment at 506 bp. Samples from both sheep and goats showed high similarity with the homologous species on GenBank. Candidatus Mycoplasma haemolamae of camels CMhl, was detected in Shalateen in 8.33% of examined camels andshowed a specific fragment at 310pb. Haemoplasma incidence in cats was 32% in Giza governorate. Candidatus M haemominutum (CMhm) and Candidatus M. turicensis (CMt) were detected in 16% of the examined cats and gave a specific fragments (202 bp) and (138 bp) respectively. Mycoplasma haemofelis could not be detected at all. Candidatus M. haemominutum (CMhm) showed high similarity with the homologous species on GenBank. Candidatus Mycoplasma haematoparvum (CMhp( was detected in dogs in Dkahlia governorate with 22% incidence and gave specific band at 380bp.
Conclusion: Haemoplasmas are circulating in different animal species in Egypt and PCR technique is the accurate method for detection of haemoplasma and sequencing is confirmatory to the results.

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