Detection of Salmonella Spp. in Meat and Meat Products by Culture, Biochemical and Molecular Characterization in Duhok City

Ahmed, Fatima Sedeeq; Mohammed Abdo, Jassim; Sulaiman Abdulaziz Jakhsi, Nadhim

doi:10.21608/ejvs.2023.230031.1569

Detection of Salmonella Spp. in Meat and Meat Products by Culture, Biochemical and Molecular Characterization in Duhok City

Document Type : Original Article

Authors

¹ Department pathology Microbiology, College of Veterinary Medicine, Duhok University, Iraq

² Department of Basic Science, College of Pharmacy, Duhok University, Iraq

³ Head of University of Zakho, Iraq

10.21608/ejvs.2023.230031.1569

Abstract

Current result illustrated in indicate that total of 150 samples of local and imported meat and meat products were collected from different location of Duhok province from November 2021 to August 2022, as follow ,25 samples=(16.66%) from each type of meat included beef meat, samp burger meat, chicken meat , minced meat , sausage meat and sheep meat.
The study comprised between traditional, biochemical and PCR. The percentage of Salmonella spp. isolation was 29.3% (44/150) positive samples by using the Traditional culture methods. 23.33% (35/150) positive samples by biochemical method, and 33% (33/60) positive samples by PCR method and confirm by serological.
Accuracy techniques from(Salmonella, culture items (counts) and Accuracy % from Salmonella True positive (35),true negative( 115),false positive ,positive predictive value (80.8%)negative predictive value (100%) and Accuracy94.47%, sensitivity100%, specificity(92.8%).
Salmonella spp. invA was found using the polymerase chain reaction (PCR). Salmonella isolate from meat and meat products .These Salmonella isolates appeared to possess the invA gene according to the PCR technique since DNA amplification produced one unique band (size 389 bp) when electrophoresed on an agarose gel. Only 10 isolates were sent to the Gene BankNCBI for registration of the nucleotide sequences for the 660 bp of the invA gene based on the sequencing method. This was done to diagnose the isolates on a species level, receive accession numbers (BankIt2717061 SAL.), submitted.

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