Prevalence and Characterization of Streptococcus pyogenes Isolates from buffalo milk, cattle milk and human milkers with M protein serotyping.

Mohammed, Mohammed Darwish; A. Gad-Elsaid, Wagih; D.Morgan, Sabry; M. Elshabrawy, Mona; E. Hashad, M.

doi:10.21608/ejvs.2023.212206.1513

Prevalence and Characterization of Streptococcus pyogenes Isolates from buffalo milk, cattle milk and human milkers with M protein serotyping.

Document Type : Original Article

Authors

¹ microbiology department NRC

² Department of Microbiology and Immunology, Faculty of veterinary medicine, Cairo University, Cairo, Egypt.

³ Department of Milk hygiene, Faculty of veterinary medicine, Cairo University, Cairo, Egypt.

⁴ Department of Microbiology and Immunology, veterinary research institute, NRC, Cairo, Egypt.

10.21608/ejvs.2023.212206.1513

Abstract

ABSTRACT
A total of 440 raw milk samples (apparently normal) were collected from different animal farms at Sohag governorate (368from cows and 72 from bufallos) and 55 throat swap samples collected from human milkers. All the Streptococcus pyogenes (S.pyogenes) isolates were identiﬁed by their bacitracin sensitivity, positive spy1258 detection, and positive GAS latex agglutination. Diﬀerent isolates were typed serotypically for M protein typing. Serotyping results indicated ﬁve diﬀerent serotypes, M1, M2, M3, M4, and M6, in S.pyogenes isolates, while six isolates were identiﬁed as untypeable. Some isolates were underwent polymerase chain reaction (spy1258) for confirmation and comparison between animal and human isolated strains. In addition, antimicrobial resistance was tested to eight diﬀerent antibiotics. the resistance mechanisms were determined phenotypically by the disc diﬀusion method. Resistance testing indicated highly resistance for mostly used antibiotics as erythromycin and clindamycin and found to be complete or inter mediate sensitive to Penicillin-G, Cefuroxime, Ampicillin /Sulbactam and Cefoperazone.

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