Residual Studies of Cefquinome and Tylosin against Experimentally Induced Echerishia Coli Infection In Chicken Using HPLC

Mahmoud, Reham; Abdelmageed, Shaimaa; Fadel, Mai A.

doi:10.21608/ejvs.2025.416517.3070

Residual Studies of Cefquinome and Tylosin against Experimentally Induced Echerishia Coli Infection In Chicken Using HPLC

Document Type : Original Article

Authors

¹ Pharmacology and Pyrogen Unit, Department of Biochemistry, Toxicology and Feed Deficiency, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Giza, P.O. Box.12618, Egypt

² Department of Microbiology, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Benha branch, Egypt

10.21608/ejvs.2025.416517.3070

Abstract

Antibiotic resistance is a worldwide problem either for human or animals. Antibiotics combination is an effective way to combat this resistance. The current work studied the effect of cefquinome and tylosin combination against E. coli53.Fifty-eight broiler chickens were separated into 5 groups. Group I and II were used as control negative and positive respectively. Group III, IV and IV were all infected with E. coli 53 but cefquinome treated, tylosin treated and the combination; respectively. Cefquinome and tylosin were extracted from tissues matrix and detected by validated reversed phase high performance liquid chromatography (RP-HPLC) technique. The technique used determined cefquinome and tylosin with low quantification limits 30.3 ppb and 24.6 ppb; respectively. Moreover, the method achieved high recovery percentage reached 99.4% for cefquinome and 99.1% for tylosin. The minimum inhibitory concentration (MIC) was 0.025mg/L for cefquinome against E. coli 53 while tylosin recorded a resistance level which was 16mg/L. Fraction inhibitory concentration (FIC) index was measured as 0.85 judged the combination effect as additivity. The tissues residues recorded higher levels of cefquinome and tylosin in Group V than Group III and IV; respectively resulted in prolongation of the withdrawal time of cefquinome for kidney and liver tissues to 5 days after cessation of the combination administration (Group V). These results revealed the additive manner of cefquinome and tylosin combination against E. coli 53 resulted in more recorded levels of tylosin especially for kidney and liver and elongation of the withdrawal period of cefquinome.

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