Molecular Detection and Epidemiological Insight into Peste des Petits Ruminants Outbreaks in Small Ruminants in Giza, Egypt

Safwat, Mahmoud; Etman, Rabie; Karam, Reham; Khodeir, Mohamed

doi:10.21608/ejvs.2025.399518.2938

Molecular Detection and Epidemiological Insight into Peste des Petits Ruminants Outbreaks in Small Ruminants in Giza, Egypt

Articles in Press

Document Type : Original Article

Authors

¹ Infectious disease department, faculty of veterinary medicine , Cairo University

² Infectious Diseases Department, Faculty of Veterinary Medicine, Cairo University, Egypt.

³ Virology department, faculty of veterinary medicine, Mansoura University, Egypt. Virology department, Weqaa central lab, Weqaa Canter, MEWA, Kingdom of Saudi Arabia

⁴ veterinary serum and vaccine research institute

10.21608/ejvs.2025.399518.2938

Abstract

Peste des petits Ruminants (PPR) is a highly contagious viral disease affecting mainly goats, sheep and wild ruminants and considered as a major obstacle in small ruminants production. Four migratory flocks of sheep and goats in Giza governorate (Moatamadia village, Zenien, Saft El-Labn and Kafrberak El-Khiam) and 5 sheep admitted to the clinic of faculty of veterinary medicine, Cairo University were suspected to be affected with PPR based on field diagnosis (case history, clinical and Post mortem (P.M.) examination) during the period from October 2014 to November 2014. A total number of 40 samples (21 buffy coats, 18 nasal swabs and one spleen tissue) were obtained from affected animals for confirmation of this suspicion by virus isolation on Vero cells and RT-PCR using primers directed to the highly conserved sequence in nucleoprotein gene of PPRV. The total number of samples which gave positive results were 15 and 37 samples by virus isolation (VI) and RT-PCR, respectively. These results confirmed that the causative agent of these case series was PPRV. Also, it was hypothesized that the disease is endemic in Egypt with regular flourishment in the period around Al-Adha festival and this hypothesis required further analysis.

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