Cryoprotective Effects of Royal Jelly Ethylene Glycol Diluent on Buffalo Bull Spermatozoa

Elsheshtawy, Reda

doi:10.21608/ejvs.2025.301612.2225

Cryoprotective Effects of Royal Jelly Ethylene Glycol Diluent on Buffalo Bull Spermatozoa

Articles in Press

Document Type : Original Article

Author

Reda Elsheshtawy

National research centre

10.21608/ejvs.2025.301612.2225

Abstract

The target of this research was to measure the influence of Tris-extender augmented with a combination of Royal Jelly (RJ) and Ethylene glycol (EG) on buffalo sperm preservability. Collective (February –may 2023) buffalo bull semen was extended in Tris extender (Tris with Zero RJ and Zero EG (TCFYG) was reserved as a control. EG was supplementary in the Tris diluent at a concentration1.5% and RJ was added at concentrations (0.05, 0.1, 0.2, 0.3 or 0.4%) . Tris containing EG and the diverse RJ concentrations were reserved as investigational. Diluted semen samples were exposed to semen freezing program. Semen estimation including motility, alive%, abnormality%, intact sperm membrane and acrosome status have been carried out for both refrigerated and frozen-thawed spermatozoa. Regarding Tris Royal jelly Ethylene glycol (TRE) Post- cooling, semen quality was significantly higher in TRE₁(Tris containing 0.05% RJ) andTRE2 (Tris containing 0.1% RJ). Post freeze-thawing, sperm forward motility was noticeably increased in TRE₄(Tris containing 0.3% RJ) relative to the control and the other applied RE concentrations. The entire used concentrations of RE considerably elevated the spermatozoal membrane flexibility (HOST) in comparison with the control.

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