Document Type : Original Article
Authors
1
Anaerobic bacteria department, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Agricultural Research Center (ARC), 131, Cairo, Egypt
2
Anaerobic Bacteria Vaccines Research Department, Agriculture Research Center (ARC), Veterinary Serum and Vaccine Research Institute (VSVRI) Cairo, Egypt
3
Anaerobic Bacterial Vaccine Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC), Cairo, Egypt.
4
Anaerobic Bacteria Vaccines Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI) Cairo, Agriculture Research Center (ARC), Egypt
5
Anaerobic Bacterial vaccine department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo
6
Anaerobic Bacteria Vaccines Research, Department, Veterinary Serum and Vaccine Research Institute (VSVRI) Cairo, Agriculture Research Center (ARC), Egypt
Abstract
Clostridium bacteria are found in soil and in the guts of animals. They are anaerobic. They create a wide spectrum of poisons that cause a variety of diseases, some of them are invasive and others not invasive, but they produce a wide range of toxins responsible for varieties of diseases. Inactivated vaccines have long been used to prevent clostridial diseases in sheep and cattle, and this practice is regarded as crucial to the management of clostridial infection in many animals. This study attempts to increase the immunogenicity of the locally manufactured inactivated polyvalent clostridial vaccine in order to produce a high and prolonged duration of protection in vaccinated animals through concentration of clostridial toxoids by using ammonium sulphate and using an alternative adjuvant as Montanide Gel 01TM.. Two vaccines were prepared, vaccine no.1 (polyvalent clostridial vaccine adjuvanted with alum (traditional vaccine)) and vaccine no.2 (polyvalent clostridial vaccine adjuvanted with Montanide Gel 01TM). Both vaccines were evaluated in rabbit, sheep and cattle. After concentration of Clostridial toxoids and using Montanide Gel 01TM as a new adjuvant, the components of the polyvalent clostridial vaccine were significantly higher compared with that elicited by the traditional polyvalent clostridial vaccine. Additionaly, the dose of vaccine was reduced from 5 ml to 3 ml for cattle and from 3ml to 2ml for sheep per dose.
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