Document Type : Original Article
Authors
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1Department of Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt 2Animal House Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 22254, Saudi Arabia
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Department of Theriogenology, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt
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Department of Food Hygiene, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt
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Department of Artificial Insemination and embryo transfer research, Animal reproduction research institute (ARRI), Agricultural research Center (ARC), Giza, Egypt
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Artificial Insemination and Embryo Transfer Research Department, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), Giza, Egypt
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1Department of Theriogenology, Faculty Vet. Med., Assiut University, 71526 Assiut, Egypt 2 Faculty of Veterinary Medicine, Sphinx University, New Assiut, Egypt
Abstract
The developmental competence of buffalo oocytes often declines during in vitro conditions, primarily due to oxidative stress. The current study was designed to evaluate the effects of L-Carnitine supplementation during in vitro maturation (IVM) of buffalo oocytes. Oocytes collected from ovaries of slaughtered buffaloes were matured in IVM media supplemented with L-Carnitine at 0 (control), 0.3, 0.6, or 1 mg.mL-1, nuclear maturation, penetration, fertilization (IVF), embryo production and development were assessed. Additionally, antioxidant parameters including glutathione (GSH) level, superoxide dismutase (SOD) activity, catalase (CAT) activity, and lipid peroxidation (MDA) level in oocytes after IVM were measured. The outcomes indicated that significant improvements in oocyte maturation to metaphase II were observed in the 0.3 and 0.6 mg.mL-1 L-carnitine groups. Fertilization rates were enhanced within 0.3 and 0.6 mg.mL-1 L-carnitine groups in contrast to the control. The 0.6 mg.mL-1 L-carnitine group demonstrated significant enhancements in cleavage (2-8 cells) and morula formation. Level of GSH exhibited an elevation, and MDA level declined in L-carnitine groups, although not significantly. SOD and CAT activities were significantly elevated in the 0.6 mg.mL-1 l-carnitine treatment against the control. In conclusion, L-carnitine incorporation within IVM improves oocyte nuclear maturation, fertilization, embryo growth, and antioxidant activity in Egyptian buffalo oocytes. 0.6 mg.mL-1 emerging as the optimal concentration for overall improvements in oocyte quality and in vitro embryonic developmental competence.
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