Document Type : Original Article
Authors
1
Department of Clinical Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Sharkia, Egypt.
2
Department of Clinical Pathology, Animal Health Research Institute (AHRI), Zagazig 44511, Sharkia, Egypt.
3
Department of Clinical Pathology, Animal Health Research Institute (AHRI), Mansoura, Egypt
Abstract
Chicken infectious anemia virus (CIAV) is a very important disease of chickens with worldwide distribution, it is accountable for the huge economic losses to farmers rearing chickens, as it affect all ages of chickens and the affected birds showed marked aplastic anemia, moderate to severe atrophy of the thymus, muscular hemorrhages and immunosuppression. The present work, investigated the effect of neem liquid extract, acyclovir and haemocare on the destructive effect of CIAV, through evaluation of the biochemical, inflammatory cytokine gene expression (IL-10) and histopathological examination. The current results showed elevation in the serum ALT, AST, ALP, creatinine, uric acid and in organic phosphorus in CIAV infected chickens. In addition to hypoproteinemia, hypoalbuminemia and hypoglobulinemia with reduction in serum calcium level and inflammatory cytokine (IL-10) gene expression. Histopathologically, CIAV infected chickens showed damaging effect in the liver, kidney and thymus tissues. While, the infected groups that pre and post treated with neem liquid extract alone and or in combination with acyclovir and haemocare showed an improvement in the altered biochemical parameters, inflammatory cytokine gene expression and histopathological findings of previous tissues. In conclusion, the present results proved the effective pre and post supplementation of neem liquid extract alone and or in combination with acyclovir and haemocare in modulating the adverse effect of CIAV infection in chickens, also pretreatment with acyclovir more effective than using it in treatment.
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