In vitro Evaluation of Both Green and Chemically Prepared Silver Nanoparticles Against Trichinella spiralis Larvae and Adult Worms

Hamdan, Zeyad K.; Soliman, Mohammad I.; Taha, Hoda Abdel Halim; Khalil, Mostafa M.; Nigm, Ahmed Hamdy

doi:10.21608/ejvs.2025.345004.2592

In vitro Evaluation of Both Green and Chemically Prepared Silver Nanoparticles Against Trichinella spiralis Larvae and Adult Worms

Articles in Press

Document Type : Original Article

Authors

¹ Biology Department, College of Education for Pure Science, Tikrit University, Iraq

² Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt

³ Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt

⁴ Ain Shams University, Faculty of science, Zoology department, Abbasia- Ghalifa Mamoon street - cairo -Egypt

10.21608/ejvs.2025.345004.2592

Abstract

Trichinella infection is the leading cause of trichinosis, which is transmitted through contaminated food. Silver nanoparticles (AgNPs) have been employed as affordable treatment for Trichinella spiralis infections through experiments conducted in vivo or in vitro. This study aims to evaluate the in vitro effects of both green (G-AgNPs) and chemically (C-AgNPs) prepared AgNPs against T. spiralis. In vitro experiments include exposing the adult and larval forms, separately, to concentrations of AgNPs ranging from 10 to 100 μg/ml. The viability of the parasites was assessed with evaluating the ultrastructural changes in adult worms. In the in vitro experiment of T. spiralis, larvae or adult worms displayed 100% mortality when they were incubated at G-AgNPs concentrations above 60 µg/ml at 48 h or 100 µg/ml at 36 h, respectively. While incubations of larvae or adult worms in C-AgNPs solutions showed 100% mortality at 100 µg/ml concentration for 24 h or 36 h, respectively. This study indicates that the anti-trichinella effect of the G-AgNPs on the adult worms was higher (more mortality rates) than their effect on the larvae especially in the long term of exposure (36 h). The opposite is true in the case of chemically prepared silver nanoparticles. Besides, the adult worms showed that the anti-Trichinella effects of corresponding concentrations of both G- and C-AgNPs were similar, especially in the long term of exposure. Concerning the results of scanning electron microscopy, C-AgNPs-treated adult worms showed more obvious tremendous deformational changes whether in males or females than those of G-AgNPs.

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