Document Type : Original Article
Authors
1
Histology and Cytology, veterinary medicine, Kafr Elsheikh, Kafr Elsheikh, Egypt
2
Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafr Elsheikh University, Kafr Elsheikh, Egypt
3
Department of cytology and histology, kafrelsheikh unversity, Egypt
4
Nano medicine, Nanoscience and technology institute , Karleshiekh, Egypt
5
Department of Anatomy, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
Abstract
The problem of diabetic wounds has long been recognized as an unmet therapeutic challenge among medical society. In this study, we assess the wound healing potential of Pine Bark (PB) extract nanoparticles on diabetic wound healing through histopathological and immunohistochemical (IHC) studies. Eighteen Wister rats were divided into 3 groups (n = 6 each): control group (NC) (non-diabetic), diabetic control group (DC), and diabetic treated PB group (PN). Under general anesthesia, full-thickness wounds were conducted on the back of all the rats, and then the PB extract nanoparticles was applied topically to the wounds of the diabetic treated PB group. The percentage of wound areas was measured on days 0, 3, 6, 9, 12, 15, and 18 post-wounding. The wound granulation tissues were detected by histopathologic examination, and the CD31 micro vessels were investigated using immunohistochemistry. Our findings indicated that wounds treated with pine extract nanoparticles exhibited significantly accelerated closure rate compared to both the DC (p < 0.01) and the NC group (p < 0.05).In addition, the process of re-epithelialization was significantly improved in the PN compared to the DC (p < 0.0001) and the NC (p < 0.05). Angiogenesis was also increased in the PN wounds compared to the DC. These results suggest that Pine bark extract nanoparticles effectively promote both the rate and extent of diabetic wound healing. This study underscores the therapeutic potential of pine extract nanoparticles, as a natural product, as effective agents in enhancing wound healing in diabetic patients.
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