Document Type : Original Article
Authors
1
Surgery Department, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt.
2
Pathology & Clinical Pathology Department, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt
3
Department of Surgery, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt.
4
Department of Surgery, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt.
5
Surgery Department, Faculty of Veterinary Medicine, South Valley University, Egypt
Abstract
Tendon healing often results in the formation of mechanically and functionally inferior fibrotic scar tissue and fibrous adhesion. Therefore, the study was performed to clinically investigate the regenerative impact of platelet-rich plasma (PRP) and hyaluronic acid (HA) on donkey’s tendon injuries. Circular defects were created surgically in superficial digital flexor tendon (SDFT) at donkey’s mid-metacarpus, and animals were divided randomly into 3 groups: Control, HA, and PRP. Follow-up data were recorded for 120 days postoperatively. At 7th day postoperatively, PRP group showed an extreme statistically significant difference in the lameness score compared to the control and HA groups (P-value = 0.0001) with an absence of any evidence for lameness in all cases of the PRP group. Regarding the degree of swelling, there was only significant difference between PRP group and control & HA groups at 15th days postoperatively (P-value = 0.049 and 0.021, respectively). With transverse ultrasound images, complete filling of the SDFT defect with newly formed tissue was noticed in HA group at 4 months and PRP group at 2 and 4 months with the absence of the hypoechoic zone, which was still appearing in the control group at the day 120th, postoperatively. Using HE stain, tenocytes with good polarity were more pronounced in the PRP group, and clear orientation of the collagen bundles was observed. PRP demonstrates potential as a regenerative therapy for SDFT injuries defects in working donkeys through enhancing the proliferation and matrix synthesis of tenocytes from tendons, in addition to its anti-adhesive properties.
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