Surveillance and Molecular Fingerprinting of Multidrug-Resistant Pseudomonas aeruginosa Strains from Clinically Diseased Companion Animals and Mastitic Cows

Hassanien, Amal; Shafeek, Eman I,; Hashad, Mahmoud

doi:10.21608/ejvs.2025.367731.2698

Surveillance and Molecular Fingerprinting of Multidrug-Resistant Pseudomonas aeruginosa Strains from Clinically Diseased Companion Animals and Mastitic Cows

Articles in Press

Document Type : Original Article

Authors

¹ microbiology department faculty of vetriniry medicine cairo university Giza Egypt

² Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt

³ Professor of Microbiology, Faculty of Veterinary Medicine, Cairo University

10.21608/ejvs.2025.367731.2698

Abstract

Pseudomonas aeruginosa is a public health threat that affects various species, causing various clinical infections. This study investigated the occurrence and diversity of MDR P. aeruginosa in different samples. One hundred samples were collected from specimens of different clinically ill animal species. Bacteriological investigation resulted in the recovery of 64 P. aeruginosa isolates (64%) identified phenotypically and genotypically. The antimicrobial susceptibility test indicated 100% susceptibility for ciprofloxacin and levofloxacin. The susceptibility to moxifloxacin, clarithromycin, tetracycline, ceftazidime, ceftriaxone, and cefotaxime were conferred by 93.75%, 40.62%, 21.87%, 15.62%, 12.5% and 3.12% of the isolates, respectively. It is not worth mentioning that all isolates were resistant to ampicillin, sulbactam/ampicillin, erythromycin, nitrofurantoin, and meropenem, which belong to four different antimicrobial groups, denoting their being multidrug-resistant isolates. BamH1 restriction digestion of 16S rRNA PCR product (1500 bp) revealed two uniform segments (650 and 850 bp) in all isolates. Photogenically, Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) was applied on 20 isolates, and they were categorized into three main clusters with 50% similarity. In conclusion, P. aeruginosa is a diverse microorganism with great MDR patterns, which is considered a danger bell for public health.

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