Unique Alternative from Dietary Saccharomyces cerevisiae Enriched with Biogenic Selenium Nanoparticles for Improving Nile Tilapia Growth and Health Performance

Eltantawy, Haba; Diab, Amany; Khalafalla, Malik; Tayel, Ahmed A

doi:10.21608/ejvs.2025.346500.2576

Unique Alternative from Dietary Saccharomyces cerevisiae Enriched with Biogenic Selenium Nanoparticles for Improving Nile Tilapia Growth and Health Performance

Articles in Press

Document Type : Original Article

Authors

¹ Department of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University

² Department of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafr El-sheikh 33516, Egypt

³ Fish processing and biotechnology department, Faculty of Aquatic and fisheries sciences, Kafrelsheikh University

10.21608/ejvs.2025.346500.2576

Abstract

Nanoparticles constantly surprise us with new and innovative applications due to their unique properties; encouraging their sustainable use in aquaculture. The fortification of aquafeeds with selenium nanoparticles (Se-NPs) provides several promises for enhancing aquatic health and other aspects of fish culturing, particularly with emergence of eco-friendly methods for synthesizing these particles by microbes, which makes their use even more exciting. Present study investigated the biosynthesis of Se-NPs with Saccharomyces cerevisiae and fortification of aquafeeds with them to stimulate Nile tilapia (Oreochromis niloticus) growth behaving, blood health and oxidative status. Firstly; Se-NPs were synthesized both intracellularly and extracellularly by Saccharomyces cerevisiae as a bioreducing agent. Characterization of Biogenic Se-NPs was done by UV-Vis spectroscopy, TEM, and SEM. After that; apparent healthy (N=135) tilapia fish (12.70± 0.15 g/fish) were partitioned randomly into three groups: group(C) had basal diet feeding as a control; while the rest two groups fed basal diet containing 10g /kg S. cerevisiae (T1) and 10g /kg S. cerevisiae/Se-NPs (T2), respectively as a fine replacement of 10 % of fish meal in fish diet. The study lasted for 60 days. The results revealed significant improvements in all growth indices (e.g. percentage increase in weight gain with 134.65% in T2 compared to 84.79% in control and in Specific growth rate with 1.42% in T2 compared to 1.02% in control), hematological indices and oxidative response (increase percentage in SOD, CAT and GSH with 16.38, 12.03 and 47.62 %, respectively and 3.93% reduction in MDA percentage) in Oreochromis niloticus fed diet containing 10g /kg S. cerevisiae/Se-NPs (T2). The biochemical indices were not significantly influenced among groups especially liver (AST and ALT) enzymes, which proved the safe, nontoxic consequence of Se-NPs administered in diets (p-value > 0.05). Thus, S. cerevisiae/Se-NPs can be a good alternative for the fine placement of 10 % of fish meal in O. niloticus diets to improve growth, hematological indices, and antioxidant- related markers.

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