MiR-155 Regulation via the Synergistic Effect of Tamarix aphylla and Artemisia herba alba Extracts with 5-Flurouracil (5-FU) in Head and Neck Cancer Cell Line (HNO-97)

Al-Sharif, Mona M.; Emam, Ahmed A.; Elsharawy, Nagwa

doi:10.21608/ejvs.2025.327597.2422

MiR-155 Regulation via the Synergistic Effect of Tamarix aphylla and Artemisia herba alba Extracts with 5-Flurouracil (5-FU) in Head and Neck Cancer Cell Line (HNO-97)

Document Type : Original Article

Authors

¹ Department of Biology, College of Science, University of Jeddah, Jeddah 21589, Saudi Arabia

² Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Egypt

³ Department of Movement Science & Health, College of Sport Science, University of Jeddah, Jeddah, Saudi Arabia & Department of Food Hygiene, Faculty of Veterinary Medicine, New Valley University, Egypt

10.21608/ejvs.2025.327597.2422

Abstract

The chemotherapeutic drug 5-Fluorouracil (5-FU) is commonly used to treat head and neck squamous cell carcinoma (HNSCC). Nonetheless, the difficulties and toxicity associated with this medicine have considerably impacted its clinical use. As a result, investigating an alternate therapeutic method that decreases 5-FU toxicity while having a synergistic effect on head and neck squamous cell carcinoma (HNSCC) is a viable option for action. Tamarix aphylla (D1) and artemisia herba alba (D2), both naturally occurring products, have been shown to suppress the development of several cancer cells, including HNSCC. This study aims to look at the combined effect of D1 and D2 and 5-FU on head and neck squamous cell carcinoma (HNSCC) and determine the apoptotic activity of this combination. The viability of cells treated with monotherapy and combination therapy was assessed using the MTT test. The researchers used flow cytometry to investigate the impact of apoptosis induction and cell cycle arrest on cells. In addition, the current study used real-time PCR to examine the expression of miR-155 and a set of specified genes. We have shown that the natural compounds D1 and D2 boost miR-155. So, our study shows that D1 and D2 may have an anti-tumorigenic effect on HNO-97 cells via a novel method. Furthermore, this study sheds light on the cellular routes through which natural compounds exert their effects.

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