Document Type : Original Article
Authors
1
Department of Biochemistry, Faculty of Vet.Med. Moshtohor, Benha University, Egypt.
2
1Department of Biochemistry, Faculty of Vet.Med. Moshtohor, Benha University, Egypt.
3
1Department of Biochemistry, Faculty of Vet.Med. Moshtohor, Benha University, Egypt
4
Cairo
Abstract
Myocardial infarction (MI) is among the major reasons for passing away in the developed globe with a prevalence of about three million cases yearly. Capsaicin (Cap) exhibits antioxidant, anti-inflammatory, anti-carcinogenic, and heart-protective impacts. Aim: This trial aimed to investigate the ameliorative actions of Cap administration against rats with triggered myocardial infarction using assessment of biochemical, molecular, and histopathological changes.
Method: Rats were split up into three groups. Group I (n=15); Normal control group. Group II (n=15); Isoproterenol was administered subcutaneously to rats (20 mg/kg/day). Group III (n=15); Isoproterenol was administered subcutaneously to rats (20 mg/kg/day) and then administrated orally with capsaicin daily (1mg/kg.bw) for 30 days. Parameters Creatin Kinase-MB (CK-MB), Lactate dehydrogenase (LDH), pro-B-type natriuretic peptide (pro-BNP), Troponin-T (trop-T), Myoglobin, hs-C reactive protein (hs-CRP), Tumor Necrosis Factor Alpha (TNF-α) and Interleukine-6 (IL-6) were measured in the three groups using ELISA method. Also, Through the use of real-time Quantitative PCR, the expression levels of Matrix Metalloproteinase-9 (MMP-9), Hypoxia-inducible factor-1-alpha (hif1-α), and sirtuin-1 (Sirt-1) were measured in the three groups. Additionally, Histological examination were carried out in the heart tissue in all groups.
Results: The findings showed that rats with MI caused by isoproterenol had significantly higher blood levels of CK-MB, LDH, pro-BNP, Trop-T, myoglobin, hs-CRP, IL-6, and TNF-α in group II contrasted with the group I. Also, Cardiac tissue of isoproterenol-induced MI rats in group II indicated significant up-regulation in MMP-9 and hif1-α gene expression levels contrasted with the group I (P<0.05). Conversely, significant down-regulation in Sirt-1 expression level in group II in contrasted to group I. Various histopathological alterations were detected in heart tissue of rats treated with isoproterenol in group II as opposed to Normal control group I. Interestingly, rats treated with capsaicin in group III demonstrated marked reduction (P<0.05) in all measured biological parameters CK-MB, LDH, pro-BNP, trop-T, myoglobin, hs-CRP, IL-6 and TNF-α II in contrasted to group II. Additionally, group III indicated significant down-regulation in MMP-9 and hif1-α gene expression levels compared to group II (P<0.05). Conversely, significant up-regulation in Sirt-1 expression level in group III compared to group II. Also, Heart tissue showed improvement in pathological alterations in Cap treated group III in comparison to isoproterenol rats in group II.
Conclusion: These findings suggest that supplementation of capsaicin counteracts the negative effects of Isoproterenol, and could be used as a new alternative cardio-treated strategy at Infarction of the heart.
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