Document Type : Original Article
Authors
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1Chemistry of Natural and Microbial Products Dept., Pharmaceutical Industries Div., National Research Centre, 33 EL Bohouth St., Dokki, 12622, Giza, Egypt. 2Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies
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Department of Biotechnology and Life Sciences, Faculty of Postgraduate studies for Advanced Sciences, Beni-Suef University
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Biotechnology and life Sciences department, Faculty of postgraduate studies for advanced Sciences, Beni-suef University
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1Chemistry of Natural and Microbial Products Dept., Pharmaceutical Industries Div., National Research Centre, 33 EL Bohouth St., Dokki, 12622, Giza, Egypt.
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Chemistry of Natural and Microbial Products Department, National Research Centre
Abstract
Marine actinomycetes, especially Streptomyces sp., are known for producing diverse biologically active compounds like antifungal, antioxidant, and anticancer agents. This study aims to optimize the production of bioactive secondary metabolites by marine Streptomyces acrimycini strain MBS-HRS-EG (MYI1) ID: PP477812.1 through submerged fermentation. The study involved screening for antifungal and antioxidant activities using agar well diffusion and DPPH methods. A medium pre-screening identified the most effective medium, which was further optimized using a Plackett-Burman design (PBD) with eleven factors and fourteen runs. Statistical analysis showed significant models with a lack-of-fit of 0.212 and R2 above 0.9768 for all responses. MgSO4, sodium pyruvate, and (NH4)2SO4 were key bioactivity variables. The optimized conditions were tested in a 7.5 L bioreactor at different agitation speeds, showing higher productivity at 200 and 400 rpm. Cytotoxicity assessment revealed the efficacy of the bioactive compounds against various cancer cell lines, with IC50 values ranging from 75.8 to 108.5 µg/mL.
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