Main Compounds, Antioxidant Activity and Lethal Effect of Thymus pallescens Essential Oil on Protoscoleces of Hydatid Cysts

Kadari, Yamina; Selles, Sidi Mohammed Ammar; Kouidri, Mokhtaria; Ould Amer, Imane

doi:10.21608/ejvs.2025.327474.2421

Main Compounds, Antioxidant Activity and Lethal Effect of Thymus pallescens Essential Oil on Protoscoleces of Hydatid Cysts

Document Type : Original Article

Authors

¹ Veterinary Sciences Institute, University of Tiaret, 14000 Tiaret, Algeria.

² Biomedicine Department, Veterinary Sciences Institute, University of Tiaret, 14000 Tiaret, Algeria.

³ Faculty of Life and Nature Sciences, University of Tiaret, Tiaret 14000, Algeria

10.21608/ejvs.2025.327474.2421

Abstract

This work aimed to investigate the chemical constituents, in-vitro scolicidal, and antioxidant activities of Thymus pallescens. Hydrodistillation and gas chromatography with flame ionization detection and gas chromatography-mass spectrometry were used for the extract and determination of compounds in essential oil, respectively. The antioxidant activity of essential oil was assessed by DPPH assay and FRAP assay. Collected aseptically from the sheep livers infected with hydatid cysts slaughtered at the Tiaret abattoir, the protoscoleces used in the present study were washed previously with sterile normal saline. Essential oil scolicidal activity was carried out at the dose (4.55 and 9.1 mg/ml) during 5, 10, and 15 min. An average essential oil yield of 1.87 ± 0.30 % (w/w) was obtained in the present study. Carvacrol (57.31%) was found to be the major compound followed by γ-terpinene (14.09 %) and p-cymene (10.64 %). Moderate antioxidant activity was shown for this essential oil with an IC50 value of 1090.03 ± 153.59 μg/ml and an EC50 value of 1043.51 ± 130.86 μg/ml. All protoscoleces were killed only after 5 min of exposure to the dose of 9.1 mg/ml. Good scolicidal and moderate antioxidant activities of Thymus pallescens essential oil was shown in the present study.

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