Document Type : Original Article
Authors
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1Department of Veterinary Medicine, Faculty of Veterinary Medicine, Beni-Suef University, Beni Suef, Egypt.
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Department of Veterinary Medicine, Faculty of Veterinary Medicine, Beni-Suef University
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Faculty of Veterinary Medicine, Beni-suef University P.C.62511. Egypt
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1Department of Veterinary Medicine, Faculty of Veterinary Medicine, Beni-Suef University, 62511, Egypt
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3Desert Research Center, Cairo 11435, Egypt
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5College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
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The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070
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4The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070
Abstract
Bovine Rotavirus (BRV) remains the most recognized worldwide pathogen causing acute diarrhea in calves under one month of age. The current study was designated to estimate the prevalence of Bovine Rotavirus infections in cattle and buffalo calves in different localities in Egypt under different management systems and study the role of some risk factors associated with BRV infections as well as the role of previously infected animals as reservoirs of infection. Fecal samples of 222 diarrheic calves, 5 recently calved cows and 17 apparently healthy calves previously infected with BRV were screened for the presence of BRV antigen by the Rapid Rotavirus immunochromatographic assay (ICA). The overall prevalence of BRV infection using the ICA was 25 (11.26%). The prevalence of BRV infection was investigated in relation to different epidemiological variables including, the animal species, sex, age, breed, dam parity, areas of study, rearing system, method of colostrum feeding and pre-calving vaccination of dams. Different risk factors were proved to influence the prevalence of BRV infections in the current study. The enzootic situation of BRV infection has been supported and proved in this study in different localities in Egypt. BRV previously infected recovered calves have been proved to remain as carriers and reservoirs leading to maintenance of BRV infection in the herd. Rotavirus double-stranded RNA was extracted from the 25 fecal samples and subjected for one-step RT-PCR targeting the NSP5 gene, and two-step RT-PCR targeting the partial length VP4 gene. The presence of the viral genome was confirmed by amplification of NSP5 gene and VP4 gene with 155 bp and 856 bp expected product size, respectively. Partial nucleotide sequencing of VP4 gene and P-typing indicated that VP4 gene of BRV belongs to the P[11] genotypes.
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