Genotyping of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Bovine Mastitis

El-shenawy, Fawzia; Ahmed, Heba A.; El- Shemy, Mariam M. G.; Talayea, Asmaa

doi:10.21608/ejvs.2024.335736.2489

Genotyping of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated From Bovine Mastitis

Articles in Press

Document Type : Original Article

Authors

¹ Bacteriology unit, Animal Health Research Institute, Tanta city, Agricultural Research Center (ARC), Giza, Egypt.

² Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, Zagazig city, Egypt

³ Bacteriology unit, Animal Health Research Institute, Tanta city, Agricultural Research Center (ARC), Giza, Egypt

10.21608/ejvs.2024.335736.2489

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) has a grown impact in veterinary medicine in the last 2 decades and triggered severe complicated multi-drug resistance mastitis in dairy herds. Therefore, we isolated Staphylococcus aureus from bovine mastitis in three different governorates in Egypt, during summer and winter seasons, assessed the sensitivity and resistance profile of the isolates to different antibiotics used in the field. Also, identified mecA and coa genes in MRSA isolates and utilized PCR RFLP for genotyping of the isolates. Our results revealed an isolation rate of 53.3% (64/120) of S. aureus, with significant difference between the governorates under investigation. Isolates demonstrated high rates of multidrug resistance (MDR), with MAR index ranged from 0.57 to 1. The highest MAR index was observed in 8 (12.5%) isolates recovered in the winter season. The mecA gene was identified in (59%) of the isolates which positively harbored coa gene amplicon with three different product sizes (670bp, 430bp, and 580bp) varied according to the location of sample collection. Restriction of coa gene by AluI resulted in 3 different RFLP patterns of MRSA with pattern 1 was the most common and strongly related to MRSA isolates from Kafr-Elsheikh. In conclusion: our results identified a high rate of MDR MRSA causing bovine mastitis in Egypt due to three different genotypes based on RFLP PCR of the coa gene. Data analysis revealed genotypic relations among MRSA isolates in the same governorate without seasonal or species association.

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