Document Type : Original Article
Authors
1
1Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, 35516, Egypt.
2
Department of Botany, Faculty of Science, Mansoura University, 35516, Egypt
3
Department of Bacteriology, Mycology, and Immunology, Faculty of veterinary medicine, Mansoura University,35516, Egypt
Abstract
The objective of this work was to synthesize AgNPs using environmentally friendly processes, characterize these structures, and assess their antibacterial efficacy against Staphylococcus aureus isolated from mastitic cow's milk that was obtained from various dairy farms in Dakahlia governorate, Egypt. Totally, one hundred mastitic milk samples were cultured on Paired Parker agar media, based on morphological, cultural, biochemical characters and PCR amplification of species- specific gene. Additionally, silver nanoparticles (JCAgNPs) were synthesized using extract from Jatropha curcas, and their evaluation was done through, transmission electron microscopy (TEM), and UV-visible spectroscopy analysis, zeta potential and the Fourier Transform Infrared Spectroscopy (FTIR analysis). The generation of nanoparticles confirmed through colour changed from light yellow to brown and UV-visible spectrophotometer confirmed its production at 300 - 700 nm. The Transmission Electron Microscopy (TEM) analysis revealed that the silver nanoparticles were spherical shape with a size range of approximately 11–20 nm and have a zeta potential of 18.6 Mv. Antibacterial potential of JCAgNPs alone was assessed against the two S. aureus isolates by disk diffusion method. Furthermore, the selected isolates were then tested for the effect of JCAgNPs combined with antimicrobials: vancomycin (VA), trimethoprim / sulphamethoxazole (SXT), cefotaxime (CTX) amoxicillin/clavulanic acid (AMC).Obviously, the antibacterial activities of the selected antimicrobials were increased in the presence of JCAgNPs against S. aureus strains. JCAgNPs increased the activity of the selected antimicrobial agents by which could be applied as a novel therapeutic method against Staphylococcus mastitis infections.
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