Document Type : Original Article
Authors
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1. Animal and Poultry Production Division, Desert Research Center, 11753, Cairo, Egypt. 2. Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls), Cairo, Egypt.
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Cairo University,Faculty of Agricultur
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2Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls), Cairo, Egypt.
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2 Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls), Cairo, Egypt.
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4.Department of Biology, College of Science, Taibah University, Madinah, P.O. 344, Saudi Arabia
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2.Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls), Cairo, Egypt.
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1. Animal and Poultry Production Division, Desert Research Center, 11753, Cairo, Egypt.
Abstract
Oocyte quality and culture conditions affect in vitro embryo development. Thus, this study examined how oocyte quality and culture media affect goat embryonic development. In the first experiment, zygotes were cultured in basic (G1), synthetic oviduct fluid (SOF; G2), and GT-L™ (G3) media. Additionally, embryonic development was monitored for seven days. Morphologically, the second experiment split 730 cumulous oocyte complexes (COCs;6 repetitions) into (G1) good-quality and (G2) low-quality groups. COCs matured and fertilized in vitro, and the best medium from the first experiment was used to produce presumptive zygotes in vitro. The nuclear maturation rate was assessed by Hoechst staining. Two investigations examined embryonic development by measuring cleavage, morula, and blastocyst formation. Data from this study indicated that the embryonic development rate (cleavage and blastocyst rates) was higher in the group cultured with G-TL™ (50.56 and 42.83%, respectively) than in basic (5.96 and 2.64%, respectively) and SOF media (23.1 and 12.25% respectively). In the second experiment, the nuclear maturation rate (extrusion of first polar body (non-invasive assessment; morphology) and Metaphase II % (invasive assessment by Hoechst staining) was significantly increased (P<0.05) in good (29.79 and 46.67%) compared to bad COCs (9.33 and 13.33%). A higher proportion (P≤0.01) of oocytes with diffuse mitochondria distribution (increased level of mitochondrial aggregation around the nucleus (central) indicates oocyte maturation) was observed in good (66.67 %) than low-quality COCs (6.64%). In conclusion, selecting good quality oocytes and culturing presumptive zygotes in G-TLTM medium improved goat in vitro embryo production (IVP).
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