Document Type : Original Article
Authors
1
Medical Parasitology Department, Faculty of Medicine, Helwan University, Cairo, Egypt.
2
Department of Medical Parasitology, Faculty of Medicine, Cairo University, Egypt.
3
Medical parasitology department, faculty of medicine, Helwan university
4
Applied Organic Chemistry Department, National Research Centre, Giza, Egypt.
5
Department of Zoonotic Diseases, Veterinary Research Institute, National Research Centre, 33 Bohouth str. Dokki, Giza, Egypt
Abstract
This study was conducted to investigate the potential therapeutic effect of S-methylcysteine (SMC) used singly, in combination with pyrimethamine (P) and sulfadiazine (S) regimen, and in conjugation with metal-organic framework nanoparticles (MOFs) on chronic toxoplasmosis in immunosuppressed mice. Fifty-four mice were immunosuppressed and divided into 9 groups (G); GІ (negative control), GП (positive control), GⅢ- GⅣf (infected and treated); GⅢ (P-S), GⅣa (SMC50), GⅣb (SMC25), GⅣc (SMC25, P-S), GⅣd (SMC25-MOFs), GⅣe (SMC25, P-MOFs, and S-MOFs), and GⅣf (SMC25-MOFs, P-MOFs, and S-MOFs). The infected mice were orally inoculated with 20 Toxoplasma cysts/ mouse. Mice treatment stared Fifty days post infection for 10 consecutive days. Assessment of the therapeutic effect of each treatment model included: parasitological parameters (brain cyst burden), histopathological examination of mice brain sections, and immunological assessment of mice serum interferon gamma (IFN-Υ) levels. GⅣf exhibited the highest percentage of brain cyst reduction (70.54%) and the highest percentage of IFN-Υ levels increase (182.23%). Nevertheless, significant increase in glial cell number, an evident increase in the degree of inflammation. Drugs loaded on MOFs have anti-Toxoplasma effect on chronic toxoplasmosis in immunosuppressed mice.
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