A Recent Approach for In-vitro Evaluation of the Inactivated Rift Valley Fever Vaccine

Fayed, Noha Ezz-Eldeen; Abulmagd, Diana M.; Hassan, Atwa M.; Ahmed, Marwa; Mohamed, Amal A.; Darwish, Darwish M.; Adel, El Helw H.

doi:10.21608/ejvs.2024.287303.2063

A Recent Approach for In-vitro Evaluation of the Inactivated Rift Valley Fever Vaccine

Document Type : Original Article

Authors

¹ Rift valley fever,veterinary serum and vaccine research institute ,Abbassia,Cairo

² Rift Valley Fever Vaccine Research Department, Agriculture Research Center (ARC), Veterinary Serum and Vaccine Research Institute (VSVRI) Cairo, Egypt

³ Anaerobic bacteria department, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Agricultural Research Center (ARC), 131, Cairo, Egypt

⁴ Central Laboratory for Evaluation of Veterinary Biologics (CLEVB). Agricultural Research Center (ARC), Cairo, Egypt

⁵ Anaerobic Bacteria Vaccines Research Department, Agriculture Research Center (ARC), Veterinary Serum and Vaccine Research Institute (VSVRI) Cairo, Egypt

10.21608/ejvs.2024.287303.2063

Abstract

With the global movement toward more widespread implementation of the 3Rs principle (Replacement, Reduction, Refinement of animals use) in bioscience, substantial efforts have been made to advance alternative in-vitro potency assays thus eliminating the disadvantages of the animal-based models regarding the number of used animals, assay time, costs and variability of results. The objective of the present study is to assess the use of sandwich ELISA as an alternative in-vitro potency assay for quality control and release testing of the inactivated RVF vaccine. Three inactivated Rift Valley fever vaccine batch samples in addition to a reference sample were evaluated by both in vitro and in-vivo methods. The results demonstrated the correlation between the antigenicity measured by in-vitro potency assay and the immunogenicity assessed by the current in-vivo assays. In conclusion, the in-vitro potency assay proved to be predictive to the in-vivo immunogenicity, and can be a reliable alternative not only provide a mean for ethical research by replacing animals use but strongly impacting the assay time, cost and efforts and pave the way for adoption of the consistency approach as a strategy for vaccine quality.

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