Steroid Hormones and Inflammatory Markers in the Uterine Tissue and Follicular Fluid of Cattle and Buffalo with Uterine Bacterial Infection

Soror, Ashraf Hasan; S. Kotp, Mohamed; Sayed, Mohamed Aly; El-gabry, Mahmoud Abbas; Fouad, Ehab; El-Debaky, Hazem Ahmed; Abo El-Maaty, Amal Mahmou; Attwa, Khaled A.; Desouky, Hassan M. Mohamed

doi:10.21608/ejvs.2024.311068.2304

Steroid Hormones and Inflammatory Markers in the Uterine Tissue and Follicular Fluid of Cattle and Buffalo with Uterine Bacterial Infection

Document Type : Original Article

Authors

¹ National Research Centre

² Animal Reproduction and Artificial Insemination department, Veterinary Research Institute, National Research Centre, Cairo, Egypt

³ animal reproduction and artificial insemination, veterinary research institute, national research Centre, Giza, Egypt

⁴ Dep of Zoonosis

⁵ National Research centr

10.21608/ejvs.2024.311068.2304

Abstract

Endometritis is the most common reproductive infertility problem in dairy animals. This study aimed to investigate the relation between the histopathological characterization of endometritis and ovarian steroid hormones in both uterine tissue and follicular fluids (FF) in addition to the proinflammatory cytokines and myeloperoxidase (MPO). Uteri collected from slaughter houses were subjected to hematoxylin and eosin (H&E) routine staining and Antisyndecan-1 immunohistochemistry aids to classify the type of endometritis. Bacteria in the apex of the examined uterine horns were isolated and identified. FF and uterine tissues homogenate were used to assay E2, progesterone (P4), tumor necrosis factor α (TNFα) and MPO. Results showed that sample-species type (cow uteri, buffalo uteri, cow FF, and Buffalo FF fluids) influenced (P<0.0001) MPO, E2, and P4. Uterine (Ut) pathology (acute suppurative, acute lymphocytic, chronic suppurative, chronic metritis, and normal); the isolated bacteria; Ut Pathology × Sample-Species; Ut Pathology × bacteria; and Bacteria ×Sample-Species influenced (P<0.0001) TNFα, MPO, E2, and P4. Ut Pathology × bacteria × Sample-Species affected TNFα, MPO, and P4. In conclusions, Immunohistochemistry is a reliable method to confirm the chronicity of endometritis. Both TNFα and MPO would be reliable markers for determining endometritis. Endometritis likely altered the FF proinflammatory markers and the steroid hormones and sequentially the future fertility. Buffaloes seem sensitive to uterine pathology caused by bacteria.

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