Artificial Insemination and Embryo Transfer Research Department, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), AL haram, Giza, Egypt.
This study evaluated the possible protective role of chitosan nanoparticles (CNPs) on ram semen after cryopreservation using a Tris-egg yolk-based extender. Chitosan synthesis and characterization were done by transmission electron microscope (TEM), scanning electron microscope (SEM) and energy dispersive analysis of X-ray spectroscopy (EDAX). Semen ejaculates were collected by artificial vagina from five rams with proven fertility (two ejaculates / ram / week) for five weeks. Only good quality ejaculates were pooled and then diluted with tris extender supplemented with different concentrations of CNPs (0, 5, 15, 25, 50, and 75 μg/ml). The diluted Samples gradually cooled to 4°C over 90 min, then equilibrated for farther 2hrs in a cold Cabinet (4°C) after that packed in 0.5ml French straws, frozen on liquid nitrogen (LN) vapor for 15 minutes then stored in LN until evaluation. Post-thawed samples were examined by computer-assisted sperm analysis (CASA) recording sperm motion parameters. Additionally, the acrosome defects, membrane integrity, antioxidant activity as well as sperm ultrastructure, and DNA integrity were recorded. The results showed a significant improvement in total, rapid, and progressive motility, in addition to preserving the integrity of the acrosome, the plasma membrane, and the ultrastructure of sperm after adding (15 µg/ml) of chitosan nanoparticles. There was a non-significant increase in antioxidant activity with a decrease in the levels of lipid peroxides and no adverse effect on the DNA. It could be concluded that CNPs cross-linked with dextran sulfate were able to successfully protect the viability of frozen-thawed ram sperm by decreasing cryo-injury.
Elshamy, A. (2024). Effect of Chitosan Nanoparticles on Ram Sperm Characterisation after Semen Cryopreservation. Egyptian Journal of Veterinary Sciences, (), 1-11. doi: 10.21608/ejvs.2024.303143.2249
MLA
Ayat abd Elmaqsoud Elshamy. "Effect of Chitosan Nanoparticles on Ram Sperm Characterisation after Semen Cryopreservation". Egyptian Journal of Veterinary Sciences, , , 2024, 1-11. doi: 10.21608/ejvs.2024.303143.2249
HARVARD
Elshamy, A. (2024). 'Effect of Chitosan Nanoparticles on Ram Sperm Characterisation after Semen Cryopreservation', Egyptian Journal of Veterinary Sciences, (), pp. 1-11. doi: 10.21608/ejvs.2024.303143.2249
VANCOUVER
Elshamy, A. Effect of Chitosan Nanoparticles on Ram Sperm Characterisation after Semen Cryopreservation. Egyptian Journal of Veterinary Sciences, 2024; (): 1-11. doi: 10.21608/ejvs.2024.303143.2249