Document Type : Original Article
Authors
1
Department of Parasitology and Animal Diseases , National Research Centre, Giza, Egypt
2
National Research centre,EGYPT
3
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, 33 Bohouth Street, Dokki, P.O. Box 12622, Giza, Egypt.
4
Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, 33 Bohouth St., Dokki, 12622 Giza, Egypt.
5
Department of Parasitology and Animal Diseases, National Research Centre,Giza, Egypt
6
Cell Biology Department, National Research Centre,Giza, Egypt
7
Department of Parasitology and Animal Diseases, National Research Centre, Giza, Egypt
8
Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt.
Abstract
We aim to determine effects of BAF-EE and CLP-EE ethanol extracts on apoptotic-gene expression , hemogram and serum protein profiles in S. aureus-infected mice. HPLC was utilized for qualitative estimation of phenolic compounds in both extracts. Sixty mice were divided into non-infected Group (n=15) and a group of 45 mice that were inoculated S/C with 0.1 ml of 2 ×107 CFU of a well-identified isolate of S. aureus. On 7th dpi, mice were subdivided into 3 equal groups and were orally dosed with BAF-EE, CLP-EE and Ciprofloxacin, at dosages of 200 mg, 100 mg and 20 mg/Kg BW/daily for 5 days, respectively. All mice were clinically monitored for 4 weeks for local inflammatory reactions and symptoms of sepsis. On days 3, 7, 14, 21, and 28, two blood samples were taken from three mice in each group before being sacrificed. Blood samples were used for hemogram and serum protein assays. The expression of apoptosis-genes in liver cells was investigated via semi-qRT-PCR. The data obtained show that four phenolic compounds were identified in each extract. Erytrogram was elevated after all treatments while, leucocyte counts except monocytes were elevated in mice treated with both extracts. Total protein and globulins increased in ciprofloxacin-treated mice. Treatment with both extracts reduced the expression of pro-apoptotic genes caspase-3, Bax and p53 while, increasing the anti-apoptotic gene Bcl-2, with CLP-EE outperforming BAF-EE. The study concludes that both CLP-EE and BAF-EE seem to have antibacterial capabilities, as evidenced by their protective impact on apoptosis-related genes and blood picture.
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