Genetic Diversity of GPAM, DGAT1, and SCD1 Lipogenic Candidate Genes In Egyptian and Indian Buffalo

Sihag, Sonia; Zahran, Mohamed A; Abdel-Shafy, Hamdy; Chhokar, Vinod; Kumar, Anil; Abou-Bakr, Samy; Hassanane, Mohamed S; E. Rushdi, Hossam

doi:10.21608/ejvs.2024.302279.2234

Genetic Diversity of GPAM, DGAT1, and SCD1 Lipogenic Candidate Genes In Egyptian and Indian Buffalo

Document Type : Original Article

Authors

¹ Department of Bio & Nano Technology, Guru Jambheshwar University of Science & Technology, Hisar-125001, Haryana, India.

² Department of Animal Production, Faculty of Agriculture, Cairo University, Algammaa Street, 12613 Giza, Egypt.

³ Cell Biology Department, National Research Center, 12622 Dokki, Giza, Egypt

10.21608/ejvs.2024.302279.2234

Abstract

The objectives of the present study were to analyze polymorphisms in the glycerol-3-phosphate acyltransferase mitochondrial (GPAM), diacylglycerol O-acyltransferase 1 (DGAT1), and stearoyl-coenzyme A desaturase 1 (SCD1) genes in the Egyptian buffalo breed, and also to explore genetic diversity across Egyptian, Murrah, and Bhadawari buffalo populations. Genotyping was conducted using PCR-SSCP and sequencing techniques. Results indicate significant deviations from Hardy–Weinberg equilibrium (HWE) in the Egyptian buffalo breed for GPAM, DGAT1, and SCD1 genes, with Murrah significantly differing at DGAT1 and SCD1 genes. Bhadawari breed significantly deviated from HWE only at DGAT1 gene. Pairwise comparison between populations revealed the shortest genetic distance between Egyptian and Murrah, while the longest between Bhadawari and Murrah. Multiple SNPs were identified in the genes under study, with alterations of amino acid sequences. These findings reveal novel genetic variants with potential implications for lipid metabolism and milk quality in Egyptian buffalo. Further research is warranted to elucidate the functional significance and breeding applications of these variations.

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