Green Synthesis of Silver Nanoparticles Ag-NPs Using Tomato (Solanum lycopersicum ) Fruit Extract and Detection Them

Document Type : Original Article

Authors

1 Department of Biology, College of Science, University of Mosul, Iraq

2 Department of Medical Physics, College of Science, University of Mosul, Iraq.

Abstract

The present study was conducted on the biosynthesis of silver nanoparticles from tomato fruits extract which were  prepared from an aqueous solution mix of silver nitrate at a concentration of 1.0 and 0.2 mM with tomato fruit extract at specific dilutions. The concentration 2.0 mM at a dilution of 1:2 (10 silver nitrate solution and 20 aqueous tomato extract) gave dark orange appears as an indicator of the reduction of silver present in tomato extract and the formation of silver nanoparticles (AgNPs). Bio-synthesized silver nanoparticles were detected using ultraviolet-visible (UV) spectroscopy, which gave the best result for detection specially at the 2mM of silver nitrate solution and 1:2 dilution by the appearance of the peak of the curve at the wavelength of 263 nm with an absorbance of 1.8963 nm. These nanoparticles were also detected using the FTIR test, which indicates the presence of active groups at all concentrations used. The results showed the spectra of the biosynthetic nanoparticles with absorption bands at 1600 to 3500 cm-1, so the 2.0 mM concentration of silver nitrate solution at 2:1 dilution gave the best result for the active groups band at the H=O bond reached to 3331 cm-1 at spectrum frequency (2500- 3333) cm-1 and the band was strong and very broad because the  vibration force of water bond. The results also indicated the shape and size of the prepared Ag-NPs silver nanoparticles by scanning electron microscope (SEM) examinations, which showed a clear similarity in their size and shape with standard silver nanoparticles. Aim of study: The present study aimed to greenly synthesize AgNps using different dilutions of each of an aqueous extract of the tomato fruit and silver nitrate solutions in an easy and economical way. Also characterize the optical, structural and biological properties of nanoparticles AgNPs produced.

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