Effect of Some Lytic Enzymes Produced by Streptomyces sp. on the Camel Tick Hyalomma dromedarii Eggs (Acari: Ixodidae)

Habeeb, Salwa Mahmoud; Ashry, Heba M.; Saad, Moataza M.

doi:10.21608/ejvs.2024.283472.2016

Effect of Some Lytic Enzymes Produced by Streptomyces sp. on the Camel Tick Hyalomma dromedarii Eggs (Acari: Ixodidae)

Document Type : Original Article

Authors

¹ Parasitology and Animal Disease Department, Veterinary Research Institute, National Research Centre, P.O. Box: 12622, El Bohouth Street, Dokki, Cairo, Egypt

² Microbial Chemistry Department, National Research Centre,P.O. Box: 12622, El Bohouth Street, Dokki, Cairo, Egypt

10.21608/ejvs.2024.283472.2016

Abstract

The lethal and inhibitory effect of the chitinase and protease enzymes supplied by Streptomyces sp. NRC23; NRC16; NRC18; NRC12; NRC80; NRC 90; NRC 50; Streptomyces gisens NRRL2021 and Streptomyces pseudo griseolus, on Hyalomma dromedarii eggs were evaluated. Five concentrations from chitinase and protease enzymes were prepared. The concentrations ranged from 1/1 to 1/5 (ml enzyme / ml distilled water) were tested at room temperature. The results were obtained seven days after the treatment of the eggs. Except for Streptomyces sp. NRC23, the chitinase enzyme had the greatest inhibitory effect across all types of Streptomyces species. The lethal effect was higher than the inhibitory effect on H. dromedarii eggs. Streptomyces NRC12's chitinase enzyme had a greater inhibitory effect. The number of dead eggs treated with Streptomyces sp. NRC23 chitinase enzyme was 49±0; 40±3; 40±7 eggs at concentration 1/2; 1/3; 1/5(v/v), respectively. The number of inhibited eggs treated with chitinase enzymes of Streptomyces sp. NRC23; Streptomyces sp. NRC16; Streptomyces NRC18; Streptomyces NRC12 and Streptomyces gresius NRRL2021 were 36±8; 31±1; 48±1 and 38±8 eggs at concentration 1/2; 1/1; 1/3 and 1/1 (ml/ml), respectively. The lethal effect on eggs treated with Streptomyces pseudogriseolus protease enzyme was 39±3 eggs at concentrations 1/1; it's higher than all types of Streptomyces. Inhibitory and lethal effects on eggs treated with protease enzyme produced by Streptomyces sp. NRC 90, Streptomyces sp NRC 50, and Streptomyces sp. NRC80 were 39±5, 27±9, 31±2 eggs and 20±6, 25±11, 32±6 eggs, at concentrations 1/3, 1/4, 1/2 and 1/2, 1/3, 1/1 (ml/ml).

Keywords