Impact of Platelet Rich Fibrin Derived From Peripheral Blood and Bone Marrow (Solid Bone Marrow Aspirate Concentrate) on Skin Autograft Healing in Dogs: Comparative Study

Platelet rich fibrin (PRF) derived from peripheral blood (pb-prf) is the one of most new and commonly utilized platelet concentrate in medical therapeutic field. It has an essential role in tissue repair and healing as it is a source for a wide range of different types of growth factors, anti -inflammatory and post-inflammatory molecules. On the other hand, recently bone marrow has been used as a source for PRF to accelerate wound healing instead of arterial or venous peripheral blood source. This experiment was carried out to study the effect of bone marrow derived PRF, which also called solid bone marrow aspirate concentrate (sBMAC)) on full thickness skin autograft take, incorporation, survival  and healing,  and also to compare its  influence with effect of pb-prf . Our current study comprised 27 dogs in which skin autografting were done, and were divided into three groups. A control group, in which graft was carried out without any treatment; pb-prf group, in which graft was treated with PRF derived from peripheral blood and sBMAC group, in which graft was treated with PRF derived from bone marrow
      The site of grafting was inspected macroscopically during 28 days after graft implantation. The histopathological assessment for grafting area was achieved at 7^th, 14^th and 28^th days after operation.  Results showed that the two types of PRF had a stimulation impact in skin graft incorporation with grafted wound bed and healing, that was characterized by 100% graft acceptance, excellent cosmetic graft appearance, enhancement of granulation tissue creation and maturation, faster reepithelization, better graft survival in contrast to control group, which displayed a partial graft separation and late granulation tissue maturation, reepithelization and healing.  Besides that, the sBMAC graft treatment had shown a better healing outcome during the all phases of the graft healing process in comparison with the pb-prf. In conclusion, the sBMAC had a greater effectiveness than the pb-prf in full thickness skin autograft healing.