Isolation Endophytic Bacteria from Leaf Glands and Seeds of Ardisia crenata Plant and its Molecular Detection

Document Type : Original Article

Authors

1 department of biology, college of science ,university of mosul,mosul, country iraq

2 Biology department,college of science,Mosul university,musol city,iraq

Abstract

Isolation endophytic bacteria from leaf glands (nodules) and seeds of Ardisia crenata plant and cultured on Nutrient  agar (NA) medium individually. The results of two types of bacterial isolated were grown in light yellow after incubation for 24 hours and took a circular shape slightly convex and Gram positive. The isolated bacteria from leaf nodules sensitive  to all antibiotics used in this study, while the bacteria from seeds was resistance only to 10 µg/ml Cefotaxime and 10 µg/ml Ceftriaxone. The data of DNA sequencing analysis  of the nitrogenous bases for 16S rRNA gene after PCR technique with the global database, National Center for Biotechnology Information (NCBI), showed 99.29% similarity between sequence of leaf isolated bacteria and Bacillus megaterium strain YSBM5 registered, therefore its recorded for the first times at  Bacillus sp. JA Leaves, while 92.02% similarity between sequence of seeds isolated bacteria and Bacillus subtilis then its recorded at Bacillus sp. JA seed strain in NCBI. 

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History

  • Receive Date: 14 April 2024
  • Revise Date: 29 April 2024
  • Accept Date: 12 June 2024

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