Document Type : Original Article
Authors
1
Poultry Diseases Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Giza, Egypt
2
Department of Poultry Diseases, Veterinary Research Institute, National Research Centre, P.O. Code 12622, Dokki, Giza, Egypt.
3
Poultry Diseases Department, Veterinary Research Institute, National Research Centre, P.O. 12622, Giza, Egypt
4
Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Giza, 12622, Egypt.
5
4) Biotechnology Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), P.O. 12611, Giza, Egypt
Abstract
This study
aims to molecular characterization of the Chicken Anemia Virus (CAV) in young broilers in the Giza governorate, Egypt in 2021, assess the genetic diversity among the proliferating viruses, and vaccine strains, and evaluate their pathogenicity to improve viral management. Viral protein 2 (VP2) gene was amplified and subsequently conducted molecular-level characterization. In five out of the ten flocks sampled CAV was detected. Furthermore, the examination of the phylogenetic connections performed according to the sequences of the VP2 gene demonstrated the classification of the observed field strains within genogroups I, II, and IIIb. In silico analysis was performed on Cux-1 and the detected five strains to investigate the secondary structures of VP2 proteins. The findings indicated a significant resemblance in the distribution and quantity of α-helical and β-strand topologies. To assess the pathogenicity of the virus, two isolates from genotype III (MZ574096.1) and genotype II (MZ477000.1) were administered intramuscularly to sets of day-old, specific pathogen-free chicks. At 28 days following inoculation, the Red Blood Corpuscle count (X106µL) was (1.4 ±0.39 and 1.8± 0.12).
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