Document Type : Original Article
Authors
1
Agricultural Research Center, Giza, Egypt
2
Faculty of Agriculture, Cairo University
3
Regional Center for Food and Feed, Agricultural Research Center, Giza, Egypt
Abstract
In vitro, studies were carried out to investigate the effect of using yeast (Y), fibrolytic enzymes (FEN) and their mixture (Y+FEN ratio 1:1) on rumen fermentation using rumen fluid. Three levels of each additive were used (1, 2, and 3 g/kg diet). At each level, a sample (300 ±5 mg) of the contained clover hay (40%) and concentrate mixture (60%) was weighed into 125 mL glass bottles (six bottles per treatment) and two blank bottles. Each of these bottles was filled up with 40 ml of a mixture of rumen fluid and buffer solution (1:3 v/v). After 24 hours of incubation at 39ᵒC, in vitro, total gas production (GP), dry-matter disappearance (IVDMD), organic-matter disappearance (IVOMD), and CO2 were recorded. The results showed that by adding 2g of FEN, 3 g of Y, and 3g of Y+FEN, the concentration of Short chain fatty acids (SCFA), Ammonia (NH3-N), CO2, and GP levels increased significantly (P<0.05). Treatment 3g (Y+FEN) recorded the highest values of SCFA (1.46 mmol/g DM), NH3-N (6.96 mg/dl), and gas production (123 ml/g). The highest concentration of CO2 was detected at Y(3g), FEN(2g), and Y+FEN (1g) (67.46, 67.85, and 68.16), respectively. Significant (P<0.05) increase in the digestibility of NDF, ADF, ADL, hemicellulose, cellulose, DM, OM, CP, and CF of treatments FEN (2g) and Y (3g) and Y+FEN (2g). It is recommended to utilize yeast (3 g/kg diet) and fibrolytic enzyme (2 g/kg diet) or their mixture (1:1) at 2 g/kg diet in ruminant animal feed to create favorable rumen conditions.
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