Molecular Detection of Virulence Factor Genes in Candida parapsilosis Isolated from Subclinical Mastitis Goats and Antifungal Susceptibility in Mosul Province

Document Type : Original Article

Authors

1 Department of Biology, Collage of Education for Pure Sciences, University of Al-Hamdaniya, Al-Hamdaniya, Nineveh, Iraq

2 Department of Microbiology, Collage of Veterinary Medicine, University of Mosul, Mosul, Iraq

Abstract

This study 
aimed to isolate and diagnose Candida parapsilosis. The utilization of molecular technologies for identification and virulence gene detection leads to the discovery of Subclinical Mastitis in goats. According to the California Mastitis Take, fifty goat milk samples with subclinical mastitis appearances were collected from specific areas of Mosul province. They were examined using rapid diagnostic tests, which were confirmed by a polymerase chain reaction (PCR) assay. Candida parapasilosis was identified in 20/50 (40%) of the mastitis milk samples. In relation to molecular techniques and the consequences of PCR amplification of the 18S rRNA gene for identification of C. parapasilosis, the gene was present in 20 samples. Moreover, the Metallo-aminopeptidase gene was present in 20/20 (100%), and the alkaline phosphatase sequence gene was present in 15/20 (75%). However, alpha-glucosidase was present in 10/20 (50%) and sterol esterase genes in 8/20 (40%). Consequence, Antifungal sensitivity testing results showed that fluconazole and amphotericine B provided the most exceptional senitivity against C. parapasilosis isolates, followed by ketoconazole, itraconazole, and nystatin.
The outcome of the experiment indicates that goat milk contains many species of the Candida genus, with a significant prevalence of C. parapasilosis. This particular species may act as an opportunistic pathogen in mastitis.

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History

  • Receive Date: 14 February 2024
  • Accept Date: 07 April 2024

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