Evaluation The synergism Activity of Portunus armatus and Apium graveolens Extract as Antioxidant in Rats Exposed to Oxidative Stress

The Portunus armatus is a crab that is highly valuable economically, has a broad geographic distribution and is used medicinally. It is also regarded as seafood in several countries like Iraq. Celery (Apium graveolens) is a native medicinal plant to Europe. This plant has a Very wide range of usage and cultivation. Celery (Apium graveolens) is usually used in traditional medicine as a diuretic or anti-hypertensive agent. This study aimed to know the synergism effect of both portunus armatus and Apium graveolens extract against oxidative stress.
THE Portunus armatus is a crab that is highly valuable economically, has a broad geographic distribution and is used medicinally. It is also regarded as seafood in several countries like Iraq. Celery (Apium graveolens) is a native medicinal plant to Europe. This plant has a Very wide range of usage and cultivation. Celery (Apium graveolens) is usually used in traditional medicine as a diuretic or anti-hypertensive agent. 
The study included 15 experimental rats aged 2-3 months, weight 150-250gm .all the experiments were done in animal house/the college of veterinary medicine at the university of Tikrit. The 15 rats disterbured into 5 groups (G1 given distilled water for four weeks as  a negative control group, G2 given hydrogen peroxide at concentrations 1% with distal water  for four weeks as a positive control, orally using gavage,G3 given hydrogen peroxide at concentrations 1% with Apium graveolens extract of one ml for each rat daily, G4 given hydrogen peroxide at concentrations 1% with Portunus armatus extract 1 ml for each animal daily and G5 given hydrogen peroxide at concentrations 1% with given the both extract daily (1ml of ach one) for four weeks, respectively. At the end of experiment the animals were scarified. The blood was drawn by orb, to obtain the blood for hematological tests and the serum for biochemical tests, 
The levels of MDA increased significantly in the G2 comparative with control group and others treatment group’s p ≤ o.o5, The TAC and Glutathione peroxidase level decrese significantly in oxidative stresses group G2 P ≤0.01 and increase in alone extract comparative with the other groups,  while the level of TAC increase significantly in synergism group comparative with oxidative stress group (p≤ 0.05).
the concentration of SOD reduced in oxidative stress comparative with control group (p≤0.05), while its return to normal value in the synergism group pf both extract.
The results show a significant increase of liver enzyme and glucose level in oxidative exposure animals comparative to control group were the enzymes began to return to its normal value after treatment with both extract. High significant (p≤ 0.01) was obtained through the synergism effect.