Document Type : Original Article
Authors
1
Department of Microbiology and Immunology
2
Dept. Animal Reproduction, Vet. Res. Div., NRC
3
Department of Artificial Insemination and Embryo Transfer Research, Animal Reproduction Research Institute (APRI), Agricultural Research Center (ARC), Giza, Egypt
4
Animal Production Research institute
5
Department of Animal Reproduction
Abstract
Microbial contamination in the in-vitro embryo production (IVEP) laboratories can’t be underestimated even with the strictest IVEP laboratories. The current study aimed to determine the prevalence of bacterial contamination during the in-vitro maturation (IVM) procedure in an Egyptian veterinary IVF laboratory and to associate them with specific clinical outcomes with suggestions of what could be done to minimize the impact of such an event. The study included 134 samples that were used in the IVM procedure; normal salines, cystic ovaries, large follicles, ovarian surfaces, follicular aspirates, phosphate buffer salines, tissue cultures, inoculated tissue cultures with oocytes, and paraffin oil. The samples were examined for the presence of bacterial contamination using standard morphological, microbiological, and biochemical tests. The bacterial isolates were tested for their antimicrobial resistance profiles using the Kirby Bauer disk diffusion method. A percent of 85.1 % of the examined samples were positive for bacterial contamination. Different pathogens were detected; Pseudomonas aeruginosa 48.5%, Staph saprophyticus 12.7%, Staph epidermidis 12.0%, and 6.0% for each of Staph aureus and Shigella flexneri. Percent of resistance to penicillin, novobiocin, streptomycin, gentamicin, tobramycin, and ceftazidime were 79.0%, 77.2%, 68.4%, 54.4%, 37.7%, and 17.5% respectively. All the isolates were sensitive to ciprofloxacin except Shigella flexneri showed intermediate resistance. Pseudomonas was mainly susceptible to ciprofloxacin and ceftazidime while resistant to penicillin, novobiocin, gentamicin, and streptomycin. In conclusion, it is compulsory to apply strict aseptic techniques in each procedure throughout the IVM procedure. Using ciprofloxacin in the culture media provides better inhibition than penicillin and streptomycin.
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