Comparative Study of Royal Jelly, Turmeric and Wheat Germ Extenders on The Cryosurvivability, Sperm Resistance, Antioxidants and Fertility in Cattle Bulls

Elsheshtawy, Reda; Abd El-Aziz, Tamer I; Nasr, Soad M.

doi:10.21608/ejvs.2023.195643.1447

Comparative Study of Royal Jelly, Turmeric and Wheat Germ Extenders on The Cryosurvivability, Sperm Resistance, Antioxidants and Fertility in Cattle Bulls

Document Type : Original Article

Authors

¹ National research centre

² National Research centre,EGYPT

³ Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt.

10.21608/ejvs.2023.195643.1447

Abstract

The present investigation was conducted to improve the post- frozen thawed sperm characteristics, the sperm resistance and antioxidant status of royal jelly (TRJ), turmeric (TT) and wheat germ(TW) extenders in cattle bulls. Semen from five mature cattle bulls were used. Ejaculates were collected weekly for 12 weeks. Semen was diluted with Tris Citric Fructose EggYolk (TCFY) diluent kept as a control and other semen specimens were extended in TCFY diluents with definite concentration of turmeric extract, wheat germ extract and royal jelly to achieve concentration of sixty million spermatozoa/ml. The freezing process was carried out on the diluted semen. Semen evaluation was implemented. The results showed amelioration in post- frozen thawed sperm characteristics in the three extenders if compared to the control (TRIS) . The superior sperm resistance and viability index was recorded in the wheat germ extender(TW) .Higher total antioxidant capacity(TAC) and lower malondialdehyde(MDA) (oxidative biomarker) were observed in TW and TRJ extenders if compared to the control and TT extenders .The highest rate of conception was recorded in TW and TT extenders followed by TRJ and the lowest conception rate was observed in the control group(TRIS).It could be concluded that, the TW,TT and TRJ extenders were higher in the post- frozen thawed sperm characteristics, the sperm resistance and conception rate relative to the control and the superior was the TW extender.

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