Molecular Prevalence of Giardia duodenalis and Subtype Distribution (Assemblage E and B) in Calves in Siirt, Turkey

Aslan &Ccedil;elik, Bur&ccedil;ak; &Ccedil;elik, &Ouml;zg&uuml;r Yaşar; Ayan, Adnan; Akyıldız, G&uuml;rkan; Orun&ccedil; Kılın&ccedil;, &Ouml;zlem; Oktay Ayan, &Ouml;zge; Ercan, Kerem

doi:10.21608/ejvs.2023.192354.1440

Molecular Prevalence of Giardia duodenalis and Subtype Distribution (Assemblage E and B) in Calves in Siirt, Turkey

Document Type : Original Article

Authors

¹ Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, Siirt, TURKEY,

² Departmen t of Internal Medicine, Faculty of Veterinary Medicine, University of Siirt, Siirt, Turkey

³ Department of Genetics, Faculty of Veterinary Medicine, Van Y&uuml;z&uuml;nc&uuml; Yıl University, Van, TURKEY

⁴ Department of Basic Health Sciences, Faculty of Health Sciences, Marmara University, İstanbul, TURKEY

⁵ Özalp Vocational School, Van Yüzüncü Yıl University, Van, TURKEY

⁶ Department of Parasitology, Van Yuzuncu Yil University School of Medicine, Van, TURKEY

⁷ Department of Internal Medicine, Faculty of Veterinary Medicine, Siirt University, Siirt, TURKEY

10.21608/ejvs.2023.192354.1440

Abstract

Giardia duodenalis is a common intestinal protozoan parasite that infects domestic and wild mammals, birds, and humans, especially livestock, worldwide. Studies on the prevalence of Giardia duodenalis in calves in Turkey are very limited. The aim of this study was to determine the molecular prevalence and subtype distribution of Giardia duodenalis in calves in Siirt. The animal material of the study consisted of 100 calves of different breeds and sexes in Siirt province. Using a disposable latex glove, feces samples were taken from the rectum of each calf and put in separate fecal containers. Sex and age information was recorded for each sample collected. As a result, 5% and 9% positivity was detected by microscopic and nested-PCR methods, respectively. The prevalence was 9.68% in females and 7.89% in males (P>0.05). Prevalence by age groups was as follows: 11.32% in the 1-6 month group, 7.41% in the 7-12 month group, and 5% in the older than 12 months group (P>0.05). As a result of the sequence analysis of the five PCR-positive samples, 80% Assemblage E and 20% Assemblage B were detected. As a result of this study, in addition to Assemblage E, Assemblage B was detected as well, which also has zoonotic properties. This situation may pose a risk for breeders. To better understand the distribution of G. duodenalis assemblages in calves in Siirt, studies in larger herds are needed.

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