Effect of High Density of Birds› numbers and Bee Pollen Addition on The level of Heat Shock Protein 90 and Blood Parameters of Male Japanese Quails

Document Type : Original Article

Authors

1 Department of Physiology, Biochemistry and Pharmacology.,Faculty of Veterinary Medicine, Mosul University, Iraq

2 Department of Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, Mosul University, Iraq

Abstract

The aim of this study is to determine the role of bee pollen in improving the blood parameters and heat shock proteins 90 of male quail exposure to high floor density. The total number was (300) 7-week-old birds, distributed randomly into 6 experimental groups, for 8 weeks. The 1st group was put of 21 birds / m2, the 2nd group was put of 21 birds / m2 and bee pollen (BP) was added at a 20 g/kg feed, the 3rd group was put of 21 birds / m2 and added of BP at dose 30 g/kg feed. The 4th group was high density (HD) 75 birds/m2, the 5th group was HD 75 birds/m2 and supplemented with BP at a 20 g/kg feed, and the 6th group was HD 75 birds/m2 and supplemented with BP at dose 30 g/kg feed. The results showed HD for birds caused a significant decrease in hemoglobin concentration, packed cell volume, mean corpuscle hemoglobin concentration (MCHC), lymphocyte number, albumin level, glutathione, and a significant increase in stress index, Heterophil number, heat shock protein 90 (HSP), and alanine aminotransferase (ALT). The addition of 20 and 30 BP to HD birds resulted in a significant increase in hemoglobin concentration, lymphocyte number, and albumin, while causing a significant decrease in stress index, heterophil cells number, HSP, and malondialdehyde (MDA). It is concluded that adding BP alone did not affect the normal parameters of blood values, while adding it to quail birds that suffer from high population density improved blood parameters and reduced stress.

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