Pharmacological studies on tetracycline and tetracycline nanoemulsion formulas

S. Saber, Mohamed; Amer, Aziza M.; ElBadawy, Shymaa A.; Amer, Mohamed Mahrous

doi:10.21608/ejvs.2018.6573.1056

Pharmacological studies on tetracycline and tetracycline nanoemulsion formulas

Document Type : Original Article

Authors

¹ PhD Student in Pharmacology ,Veterinary Service Division, Ministry of Defence

² Department of Pharmacology, Veterinay medicine collage, Cairo University

³ Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University,

⁴ Department of Poultry Diseases. Faculty of Veterinary Medicine. Cairo University. Postal Code: 12211 Giza, Egypt.

10.21608/ejvs.2018.6573.1056

Abstract

The study was done to compare the pharmacokinetic and pharmacodynamics of 50 mg/kg b.wttetracycline hydrochloride (TC-hcl) and tetracycline nanoemulsion (TC-nm) formulasin rabbits and detection of their effect on stander and field bacterial strains. After oral TC concentration in plasma started to be detected at 0.25 h, reach the maximum at (0.5 h Tc-hcl) and 1 h.(TC-nm) and decline at 12 hours. Following a single i.v administration a volume of distribution V2 (0.292±0.111 L/kg) in TC-nm than for TC-hcl (0.216 ± 0.183L/kg) and was slowly cleared (0.393±0.183 L.h/kg) in Tc-nm than in TC-hcl (0.415±0.311 L.h/kg). After oral administration a rapidly absorbed with significant slowly absorption half-life t1/2 _alpha(0.550±0.090 h and 0.176±0.058 h.) and elimination half-life t1/2 _beta (4.215±1.661 h. and 1.58±1.447 h.) with higher calculated Cmax of (4.215±1.661 μg/ml and 1.58±1.447 μg/ml) achieved at prolonged calculated tmax (0.759±0.149h. and 0.356±0.305 h.) in TC-nm than in TC-hcl, respectively.
The value of TC-hcl and TC-nm MIC was the same for Staph. Aureus 6538, Staph. Epidermidis12228 ,E.coli 8739 were 0.14, 0.8 and 0.12 µg , respectively, and interpreted as sensitive. Field sensitive Corynebacterium, E.coli , S. typhimurium, S. enteriditisand Staph.leutus isolates MIC value was 1.4, 8, 2, 1.6 and 2 µg for , respectively. Tetracycline resistant2Staph.scuiri (18 and 16µg) and 2 Staph. xylosis ( 18 and 6µg).

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