Genetic Characterization of Methicillin Resistance Staphylococcus aureus Isolates of Poultry and Human Origin

Saleh, Mohamed; El Hady, A.M.M.; R. Mohamed, Sahar; El-Shafei, A. A.; El-Shafei, Maher

doi:10.21608/ejvs.2021.95036.1292

Genetic Characterization of Methicillin Resistance Staphylococcus aureus Isolates of Poultry and Human Origin

Document Type : Original Article

Authors

¹ Bacteriology Department , Animal Health Research Institute, Dokki , Giza, Egypt

² Department of Medical Microbiology Faculty of Medicine Zagazig University, Egypt

10.21608/ejvs.2021.95036.1292

Abstract

Our study applied on 160 collected samples, from poultry and human patients. Infection percent of human skin, poultry bumble foot and human nasal swab and Diabetic foot swab were 66% , 10% , 67.5% , 37.5% respectively. Methicillin resistant Staph aureus (MRSA) were 83.3% and 50 % from poultry and human patient respectively, mec A gene is detected by 100% on samples of poultry and humen origin. Whereas blaZ and spa gene and also, 83.3% and 50% respectively either from human or poultry samples respectively. Methicillin Resistance S. aureus (MRSA) strains isolated from human nasal swabs was 80 % . PCR technique was used for mec A , blaZ ,and Spa gene detection. PCR results were identical to disc diffusion method in 11 isolates out of 15 ( 73 %) isolates from poultry and human samples.

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