Prevalence and Molecular Characterization of Enterotoxin- and Antibiotic Resistance-Encoding Genes in the Methicillin-resistant Staphylococcus aureus Recovered From Poultry Meat

Saadati, Amirhossein; Mashak, Zohreh; Yarmand, Mohammad Saeid

doi:10.21608/ejvs.2021.48755.1202

Prevalence and Molecular Characterization of Enterotoxin- and Antibiotic Resistance-Encoding Genes in the Methicillin-resistant Staphylococcus aureus Recovered From Poultry Meat

Document Type : Original Article

Authors

¹ Doctor Veterinary Medicine, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran.

² Department of Food Hygiene, Karaj Branch, Islamic Azad University, Karaj, Iran.

³ Department of Food Science and Engineering, Faculty of Agriculture, University of Tehran, Tehran, Iran.

10.21608/ejvs.2021.48755.1202

Abstract

Enterotoxigenic methicillin-resistant Staphylococcus aureus (MRSA) is considered as one of the common foodborne pathogens. The existing research was performed to investigate the distribution of enterotoxigenic and antibiotic resistance genes of MRSA bacteria that are isolated from raw poultry meat samples. Two-hundred and sixty poultry meat samples were cultured and MRSA bacteria were recognized using cefoxitin and oxacillin susceptibility test. Antibiotic resistance and enterotoxigenic gene profiles were studied using PCR test. Prevalence of MRSA amongst poultry samples was 5%. Chicken (12%) had the highest prevalence rate, while ostrich (1.66%) had the lowest. Total distribution of sea, seb, sec, sed and see enterotoxin encoding genes were 50%, 25%, 8.33%, 75% and 8.33%, respectively. BlaZ (100%), aacA-D (58.33%), tetK (58.33%), msrA (58.33%) and dfrA1 (50%) were the most regularly detected antibiotic resistance-encoding genes. Concurrent presence of enterotoxins and antibiotic resistance-encoding genes in MRSA bacteria stipulates important public health matters regarding the consumption of contaminated chicken, turkey, quail and ostrich meat.

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