National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249120180601“Osteo-protective effect of Curcumin; Fenugreek and their combination on ovariectomized female rats”.112542610.21608/ejvs.2018.2312.1024ENNesma Mohammed EsmatAbo El-NasrDepartment of Pharmacology, National Research Centre, Cairo, EgyptEzz EddinEl-densharyDepartment of Pharmacology and Toxicology, faculty of Pharmacy, Cairo University, Cairo, EgyptSawsan Salah EldenMahmodDepartment of Pharmacology, National Research Centre, Cairo, EgyptSalwa MohamedNofalDepartment of Pharmacology, National Research Centre, Cairo, EgyptJournal Article20171214THE MOST common type of osteoporosis is the bone loss associated with ovarian hormonal deficiency at menopause. It is characterized by an imbalance between bone resorption and formation, resulting in a decrease of bone mass and a deterioration of bone microarchitecture. Purified curcumin, Fenugreek extract and their combination were evaluated for improvement of bone loss on ovariectomized rats as a model of postmenopausal osteoporosis. 60, female Wistar rats weighing 130150-g were ovariectomized (OVX) except sham-operated (SH) group. Rats fed a soya bean deficient diet and randomly divided into 5 groups. After 3 months of ovariectomy, SH group and OVX control group was received 1 % DMS0 (1 ml/kg P.O.), while, other OVX groups were received curcumin (100mg/kg p.o.), alcoholic fenugreek extract (200mg/kg p.o.) and their combination (Received the same doses of drugs). Fumer, tibia and total bone mineral density showed significant decrease in OVX control vs. sham control, while the combination of curcumin and fenugreek showed significant increase compared with OVX control group. Data obtained showed that curcumin, significantly, decreased the bone loss. In addition, curcumin combined with alcoholic fenugreek extract showed a synergistic effect that consequently improved both bone turnover and bone strength.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249120180601Effect of European seabass ( Dicentrarchus labrax ) fry source (Wild, NIOF and GAFRD K21) on its growth performance and physiology1323542710.21608/ejvs.2018.2430.1026ENAhmedSalemNational Institute of Oceanography and Fisheries (NIOF), Ministry of Scientific ResearchNagyEl-BaramawiDepartment of Animal and Fish Production,
Faculty of Agriculture (Saba Basha),
Alexandria University.NaderAbdelsalamDepartment of Agricultural Botany,
Faculty of Agriculture (Saba Basha),
AlexandriaUniversity.Mohamed SobhyAbokadahAgriculture AlexandraJournal Article20171231THE present study aimed to evaluate the impacts of different sources on the European seabass (D. labrax) fry growth and physiology. One thousand and five hundreds fry from 3 sources were transferred to the lab., the 1st fry group was collected from the wild habitat in El- Meadya region, El- Behira Gov. from the wild fry capture fisheries. The 2nd fry group was 94 (dph) day post hatching produced in the Marine NIOF Hatchery, El- Anfoushy from induced spawning and the 3rd group fry was 94 dph produced in the Marine GAFRD Hatchery from induced spawning. Fry samples were randomly collected to statistically determine the length, weight growth and physiology performances. The comparative effects of European seabass (D. labrax) fry sources (wild and two hatcheries) and their impacts on length and weight growth parameters, total length, standard length, body width, total weight, gutted weight, hepatosomatic and vesrosomatic indexes. The wild collected fry achieved the best significant (P < 0.05) results in all this growth parameters while the NIOF marine hatchery produced fry achieved the best significant (P < 0.05) results in all these physiology parameters. In conclusion, this study explained why fish farmers prefer wild collected European seabass (D. labrax) fry than hatchery produced fry. Egypt marine hatcheries need more efforts and technology transfer to help marine aquaculture and fisheries development, that need ranching and restocking with hatchery produced fry.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249120180601Protective Effects of Moringa oleifera extract on Isoniazid and Rifampicin Induced Hepatotoxicity in Rats: Involvment of Adiponectin and Tumor Necrosis Factor-α2534563810.21608/ejvs.2018.2349.1025ENMohamed FayedAbd Elhameedpharmacology department, medical division, national research centreAbeer Abdallah AliSalamaPharmacology Department National Research Centre, Giza, Egypt.Taha MohamedAttiaPharmacology Department, Faculty of Vet. Med., El-Sadat University, Minoufiya, EgyptSeham AbdelsatterElbatranPharmacology Department National Research Centre, Giza, EgyptIsmaeil El KassabeyIsmaeilPharmacology Department National Research Centre, Giza, EgyptAzzaHassanPathology Department, Faculty of Vet. Med., Cairo University, Giza, EgyptJournal Article20171219H EPATOTOXICITY is the major health problem that is a global concern worldwide especially in Egypt because the liver injury is one of the most ten disease leading to death. There are many factors leading to hepatotoxicity as antibiotics like Isoniazid (INH) and rifampicin (RIF) which are the first line remedies in treatment of tuberculosis. The present study evaluates the possible hepatoprotective effects of Moringa oleifera against the experimentally induced hepatotoxicity with INH/RFP in rats. Forty eight rats (200-250g) were allocated into six groups (8 rats in each group), and treated as follow: group I: received normal saline orally, group II: received INH/ RIF (50 mg/Kg/day of each)for 28 days orally, group III: received Silymarin (50 mg/kg/day) + INH/ RIF for 28 days orally, group IV, V, VI: received Moringa oleifera ethanolic extract (MOEE) (250, 500 and 1000 mg/Kg/day) + INH/ RIF for 28 days respectively. Co-administration of Moringa oleifera with INH/ RIF reduced elevated serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and (bilirubin total &direct) levels but also decreased the elevated malondialdehyde (MDA) & tumor necrosis factor-α (TNF-α) contents in liver homogenate. Moreover, administration of Moringa oleifera increased glutathione peroxidase (GPX) & adiponectin activity. Microscopic examination of Moringa olifera extract administration revealed reduction in the severity of liver damage. It is concluded that Moringa olifera might be considered as adjuvant drug in treatment of liver disorder and or as hepatoprotective therapy with anti-tubercular drugs.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249120180630Factors Affecting the Secondary Sex Ratio of the Iranian Raeini Goats3541800010.21608/ejvs.2018.2549.1027ENShahabodinGharahveysiDepartment of Animal Science, Faculty of Agriculture, Islamic Azad University-Qaemshahr Branch, Qaemshahr, Iran0000-0002-1069-0215PeimanHamidiDepartment Of Animal Science, Faculty ofAgriculture, Islamic Azad University-Qaemshahr Branch, Qaemshahr, IranRohullahAbdollahpourDepartment Of Animal Science, Faculty ofAgriculture, Islamic Azad University-Qaemshahr Branch, Qaemshahr, IranAbbasAbbasiDepartment Of Animal Science, Faculty ofAgriculture, Islamic Azad University-Qaemshahr Branch, Qaemshahr, IranJournal Article20180107Commonly,the secondary sex ratio - the animal proportion of the same gender in the population at birth- is equal. This study aimed to identify the environmental and genetic factors affecting the secondary sex ratio of the Iranian Raeini goats. The data from 13563 goats included 7752 males and 5811 females were used to evaluate the effect of genetic and environmental factors by fitting 10 different animal models. In these models, the effects of herd-year of birth, birth type, province, maternal age, permanent environmental (dam and sire) and genetic factors (such as additive, sire, maternal and maternal grandsire) were included. The results showed that the effect of the herd-year of birth and the birth month on the secondary sex ratio were significant (P < 0.001), while the effect of the province, birth type and maternal age were not significant (P > 0.05). By studying the animal models, it could be concluded that the contribution of the genetic factors contributing to the variations of the secondary sex ratio were negligible.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249120180601Compare the chick length and weights of the Ross broiler breeder in different ages and farms4347800110.21608/ejvs.2018.3069.1030ENShahabodinGharahveysiDepartment of Animal Science, Faculty of Agriculture, Islamic Azad University-Qaemshahr Branch, Qaemshahr, Iran0000-0002-1069-0215TaherAsadi KenariDepartment of Animal Science, Faculty of Agriculture, Islamic Azad University-Qaemshahr Branch, Qaemshahr, IranJournal Article20180301reeder flock age affects the quality and quantity of eggs and chicks. The purpose of this study was to investigate the effect of breeder flock age on egg (quality and quantity) and weight and height of hatched chicks. In recent study 271 fertilizable eggs were selected randomly from 3 farms of broiler breeder. Broiler breeder eggs were used when they were 30 (young), 36 (peak), 43 (after peak), 53 (old), 60 (very old) and 82 (molt) weeks. Collected eggs after hatching were stored during 3 to 4 hours at ambient temperature. Quantitative traits such as weight of chick and chick height were considered. The effect of ages and farms on the chick weight and chick height were significant (P < 0.001). The chick weight increased until 53 weeks then decreased. The chick height increased with age increment. According to limitation of researches about studying the effect of age of broiler breeder flock on the chick production, it is needed to carry out a research to eliminate unknown points. Since the majority of poultry have no sufficient information about the quality of chick and the best age of broiler breeder for buying chicks it could be answered to many questions using the results of this research.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249120180601Pathogenicity of Coagulase Negative Staphylococcus chicken isolates to 10 Days Old Broiler Chickens4957800210.21608/ejvs.2018.3280.1035ENMariam HussienShokryFaculty of Veterinary medicine, Cairo UniversityMohamedBosilaDept of Poultry. Diseases, Vet. Res. Division, National Research centreWafaa A.Abd El-GhanyProfessor of Poultry Diseases
Faculty of Veterinary Medicine, Cairo University0000-0003-1686-3831Ismail AbdelhafeezRedwanProfessor and head of Bacteriology , Mycology and Immunology Department, Faculty of Veterinary Medicine, Bani-suif UniversityMohamed MahrousAmerPoultry and Rabbit disease Department/Faculty of veterinary medecine / Cairo University0000-0001-8965-7698Journal Article20180322 One hundred and sixty, 1-day old broiler chicks were grouped into 4 equal groups, at the 10<sup>th</sup> day birds of groups 1- 3 were s.c inoculated with 0.5 ml containing y 1.5x 10<sup>8</sup> of <em>S.xylosis, S. sciuri </em>and <em>S.lentus</em>; respectively and group 4 was noninfected control.
Clinical signs in infected groups started at 2-3dpi as general signs. Signs disappear in Ciprofloxacillin treated subgroups 24 hr post treatment and lasted to the 7<sup>th</sup> day in non treated.
Average body weight gain in <em>S.xylosis</em> infected non treated was the highest (813.90 gm), followed by <em>S. scuiri</em> (778.50gm ) and 773.75 in <em>S. lentus</em> infected treated . FCR was the highest in control (1.69 treated and 1.74 non treated) followed by <em>S. scuiri </em>infected (1.81 non treated and 1.82 treated) and the lowest 1.94 was in <em>S.lentus</em> infected non treated. <em>S. sciuri </em>was reisolated from intestine and spleen (5<sup>th</sup> dpi) and from intestine (7 and 10 dpi). While <em>S.lentus</em>was reisolated from intestine , liver and spleen (3<sup>rd</sup> dpi) ; from intestine and spleen (5<sup>th</sup> dpi) and intestine (10<sup>th</sup> dpi).
Histopathological lesion was recorded in infected group as hemorrhages with sinusoidal dilation, focal areas of vacuolar degeneration , fatty degeneration and shrinkage of hepatocytes in liver, necrotic changes of lymphocytes and vacuolion of corpuscle in spleen. Leucocytic infiltration , degeneration and necrosis of epithelium surface and intraepithelial as well as submucosal leucoytic infiltration were seen in intestine.
In conclusion the injected organisms induce mild subclinical disease with recording of histopathological lesions in liver, spleen and intestine. This area needs more investigation to explore pathogenicity of CoNS in chickens.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249120180601Molecular detection of antibiotic resistance genes in identified of Coagulase Negative Staphylococci from chickens flocks and hatcheries in Egypt.5970800810.21608/ejvs.2018.3258.1034ENMariam HussienShokryFaculty of Veterinary medicine, Cairo UniversityIsmail AbdelhafeezRedwanProfessor and head of Bacteriology , Mycology and Immunology Department, Faculty of Veterinary Medicine, Bani-suif UniversityMohamed MahrousAmerPoultry and Rabbit disease Department/Faculty of veterinary medecine / Cairo University0000-0001-8965-7698WafaaGhanyProfessor of Poultry Diseases
Faculty of Veterinary Medicine, Cairo University0000-0003-1686-3831Journal Article20180321The collected 942 samples (6 organ/bird) from 157 birds taken from 34 broiler chicken farms aged 2- 31 days including 13 and 21 clinical diseased and apparently health flock as well as 221 hatchery samples. Samples were tested for isolation of Coagulase negative staphylococcus (CoNS).<br /> Results of isolation from chicken flocks with clinical signs are 9/13(69.23%) were positive. Positive samples (11/354, 3.11%) including : 3liver, 4 kidney, 2 intestine, 1 air sac and 1 nasal sinus. Out of apparent healthy flocks 8/21 (38.95%) were positive. Positive samples (15/ 588, 2.55%) including 3 liver, 2 kidney, 4 intestine, 3 lung, 2 air sac and 1 nasal sinus. Results showed that 15 positive flocks out of 34 flocks (34.09%) with 26 Staphylococcus isolates from 942 samples (2.77%) including 6 liver, 6 kidney, 6 intestine, 4 lung, 2 air-sacs and 2 nasal sinus with rate of 3.82%, 3.82%, 3.82%, 2.55%, 1.27% and 1.27%; respectively. Also12 isolates out of 26 (46.15%) were CoNS include 8 S. xylosus (75 %), 2 S. scuiri (16.67%) and 2 S. lentus (16.67%).<br /> Hatchery samples reveals the isolation of 26 staphylococcus isolates (11.76%). The tested 108 fertile eggs and dead in shell embryos resulted in 14 and 12 isolates in rate of 12.96% and 13.79%; respectively. Ten isolates were CoNS (38.46%) and represented 4.52% out of total samples including 8 S. xylosus and 2 S. scuiri. Eight S. xylosus was 6 (5.55%) from infertile eggs and 2 (2.29 %) from dead in shell, While the 2 S. scuiri (1.85%) were obtained from infertile eggs. <br /> The tested CoNS isolates showed 100% resistance to Oxytetracycline 30 µg/ml (T30), Trimethoprime + Sulphamethexole 2.25/23.75 µg/ml (SXT) , Calindamycin 2 µg/ml (DA) and Oxacillin 30 µg/ml (OX). All isolates were 100% susceptible to Vancomycin 30 µg/ml (VA) and 90% susceptibility to Enrofloxacin 5 µg/ml (ENR). Multidrug resistance was detected in form of resistance to 9, 4 and 5 out of tested 13 antibiotics in 2 S.lentus , 5 S. scuiri and 15 S. xylosis; respectively.<br /> Ten isolates were tested for the presence of 7 resistance gens including: mecA , tetK, blaZ , kan , ermC, icaD, bab gene. Seven isolates from the tested 10 (70%) having 4 resistance gens. The most detected gens are mecA tetK, blaZ and ermC where it was detected in 90, 80, 60 and 90% respectively. Kan , icaD and bab gens were detected in rate of 30.0 and 0 %; respectively.<br /> In conclusion: CoNS could isolated from healthy and diseased chicken flocks as well as from chicken hatchery. The obtained isolates were multidrug either phenotypic and /or genotypic resistant. Good hygienic measures in both chicken farms and hatchery with monitoring of drug resistance of CoNS those act as source for resistance genes to bacterial pathogens and their importance to the poultry and public health are recommended .National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249120180630Genetic evaluation of survival traits in the Holstein dairy cows of Iran7174884010.21608/ejvs.2018.3070.1031ENSoheilMirhabibiDepartment of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, IranNasserKashanDepartment of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, IranShahabodinGharahveysiDepartment of Animal Science, Faculty of Agriculture, Islamic Azad University-Qaemshahr Branch, Qaemshahr, Iran0000-0002-1069-0215Journal Article20180301The longevity is an important functional trait for dairy cow breeders. The aim of this study is genetic evaluation of survival traits in the Holstein dairy cows of Iran. A data set of information from the Iranian Holstein dairy cows was used for this study. The culling date of 36340 animals from 65 herds recorded in 1993-2014 was used. Lifespan and Length of productive life (LPL) were analyzed for longevity. Functional productive life (FPL) was estimated. Production traits consisted of first lactation milk and fat yield. The (Co) variance, heritability and genetic correlation were estimated with multiple-trait REML using an animal model. The mean of lifespan and length of productive life were estimated to be 954(±716) and 1710(±721) days respectively. Heritability of LPL and FPL estimated as 0.032(0.012) and 0.028(0.012) respectively. The estimates of genetic and phenotypic correlations of milk yield with LPL and FPL were -0.33, 0.20, 0.10 and 0.25, respectively. Due to the decline in length of productive life, with diagnosis of the involuntary culling in herds is possible to increase the longevity.