National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022248220171230Trial on using of some herbal extracts as promising immunoprophylaxis feed additives in cultured Oreochromis niloticus5360412710.21608/ejvs.2017.1446.1018ENWaleed Salah El-DinSolimanNRC, Vet. division, Hydrbiology Dept.0000-0002-8495-0526Journal Article20170806Medicinal herbs and some plant extracts are natural feed additives which have a great impact on fish health. The present study focused on three dietary plant extracts to determine which one could be used as immunostimulant in aqua-feed. A total 210 apparently healthy O. niloticus with an avg. body weight 30 gram were stocked in 21 glass aquaria (10 fish\group\replicate) (3 replicate/group) and fed on pelleted diet supplemented with (0.5mg, 1mg onion leaves extracts{OE}, 0.5mg. 1mg barely seed extracts{BE}, 0.5mg, 1mg rice straw extracts {RE }and 0% additive) twice daily for two months. The blood picture, innate immunological parameters and response toward pathogens were assessed. At the end of experiment there was significant increase (P < 0.05) in serum lysozyme activity, phagocytosis in groups fed OE and BE extracts while there is no effect in fish fed RE extract. Globulin level and WBCS count increased in OE and BE groups. The result of this study demonstrated that fish groups fed on OEexhibited the highest immune performance and resistance against experimental infection with Aeromonas hydrophila, coming in the second rank the BE, and to lesser extent the RE which was nearly similar to control group. Therefore, we can recommend the use of onion leaves extract in aqua-feed at 0.5mg/kg diet and\or barley seed extract at 1mg/kg diet.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022248220171201Evaluation of Buffalo Bull Semen for Some Venereal Diseases Using PCR7379434410.21608/ejvs.2017.1850.1021ENKhaledAbdel-hameedKarimaMahmoudprof Dr at National Research Center0000-0002-0482-1207AbdelazizSakrAnimal Production Research InstituteAhmedSosaNational Research Centre0000-0001-9726-2936MahmoudHasanainNational Research Center, Egypt0000-0002-3283-2309YoussefYoussefNational Research Center, EgyptMahmoud F.Nawitoanimal rep deptJournal Article20171016Artificial insemination by frozen semen is a major approach for diseases transmission. This work intended to scan the buffalo bull semen for brucellosis caused by Br. melitensis, toxoplasmosis caused by Toxoplasma gondii and Neosporosis caused by Neospora caninum using Polymerase Chain Reaction (PCR). Fifty bulls were used in this study. Semen was assessed for volume, motility, viability, and chromatin damage. Total genomic DNA was extracted from buffalo semen and amplified by PCR. The results showed that, semen samples were positive to toxoplasmosis in 6% of the semen samples, while all the semen samples were totally negative to brucellosis and neosporosis. No significant differences were found in the semen characteristics between bulls positive and that were negative to toxoplasmosis.<br /> In conclusion, the present trial suggests the opportunity of venereal transmission of T. gondii among buffaloes .However, further investigations are required using high number of semen samples from different localities of Egypt.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022248220171230Clinicohistopathological And Immunological Alterations in Egyptian Donkeys Infested by Rhinoestrus Spp. During the Winter Season6171412810.21608/ejvs.2017.1555.1019ENFaten A.MAbo-AzizaParasitology and animal diseases, Veterinary Medicine Research, National Research CentreSehamHendawyDepartment of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Cairo, Egypt.0000-0001-7122-1570ZakiAbd El-KaderDepartment of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.SamahOdaDepartment of Pathology, Faculty of Veterinary Medicine, Alexandria University, Edfina, EgyptAmiraEl-NamakyDepartment of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Cairo, Egypt.Journal Article20170823Little information is available about the homeostasis and alterations appeared against infestation by nasal myiasis caused by Rhinoestrus spp. larvae in Egyptian donkeys. Therefore, the present work aimed to investigate the hematological and biochemical profiles associated with histopathological features and immunological response developed against Rhinoestrus spp. larvae infestation in donkeys in the winter season. The results revealed that 40 out of 80 examined donkeys were infested by nasal myiasis. IgG values were higher (P< 0.01) in infested donkeys (IND) than non-infested ones all over the winter months while the highest mean larval burden was observed in December (11.6 larvae/animal). Neutrophils% in (IND) increased (P<0.01) during December and February. However, an elevation in lymphocytes% (P<0.05) was observed in (IND) during December and January while eosinophils% increased (P<0.01) in all winter months. These results paralleled to the nasal fluid cytological profile in which lymphocytes%, neutrophils% and eosinophils% in (IND) increased (P< 0.05) in December, January and February. Glutathione peroxidase, superoxide dismutase and catalase activities in (IND) were declined (P<0.01) in all winter months. Interleukin-2 (IL-2) and tumor necrosis factor- α (TNF-α) elevated (P<0.05) in December and January. The histopathological picture of turbinate bones, olfactory bulb and meninges reflected the blood and nasal fluid cytological picture as there was lymphocytes infiltration, meningitis and satellitosis. The results indicated that Rhinoestrus spp. infestation in donkeys might affect the vital and immunological processes. In addition, Rhinoestrus spp. infestation in donkeys could linked to oxidative stress and shifting the immune response polarization towards Th2 cytokines.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022248220171230Hormonal Profile, Antioxidant Status and Some Biochemical Parameters during Pregnancy and Periparturient Period in Dromedary She Camel8194494710.21608/ejvs.2017.2040.1022ENMaha AbdelhamidIbrahimanimal reproduction research institute
Giza, EgyptHowidaAbdelrahmanAnimal Reproduction Research InstituteHassanElmetwalyCamel research Department, Animal Production Research InstituteJournal Article20171111THIS STUDY aimed to determine the changes in the hormonal and biochemical profile of pregnant dromedary camels to provide a baseline data under Egyptian climatic conditions. Blood samples were collected from15 pregnant she-camels aged 5-11 years old just postconception and thereafter monthly during gestation period and during periparturient period (at days 15, 7,4and 1prepartum,at parturition and at days 1, 4, 7 and 15 postpartum).Progesterone level increased significantly (P<0.05) after conception and during the1stand the 2ndtrimester of pregnancy then declined significantly (P<0.05) during the 3rd trimester. Meanwhile its level decreased significantly (P<0.05) during periparturient period. Cortisol level significantly (P<0.05) increased at the 3rd trimester and continued increasing significantly during prepartum period with a maximum level on the day of parturition after that it was markedly decreased during the few days postpartum to day 15 postpartum. Thyroid hormones levels were significant (P<0.05) decreased in their levels from early to late gestation then increased significantly (P<0.05) from day of parturition to day 15 postpartum. Total protein and albumin were significantly low (P<0.05) in the 2nd and the 3rd trimesters than the first one with no significance alterations in globulin levels. Total antioxidant, glutathione and ascorbic acid levels were lower in the 3rd trimester than others, whereas the levels were relatively low in prepartum than 15 days postpartum. Malondialdehyde concentration was significantly increased (P<0.05) during late pregnancy period as a result of the physiological stress of pregnancyNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022248220171201Some studies on strangles in Cairo and Giza governorates9599362410.21608/ejvs.2017.1308.1017ENOsama MahmoudMohamedinfectious disease department, college of veterinary medicine, cairo university, Cairo, EgyptJournal Article20170711The present work was designed to study the relation between the rate of strangles infection in equine and seasonal incidence in Cairo and Giza governorates, beside the isolation and identification of Streptococcus equi. Seventyeight horses of different ages have been studied for S.equiinfection along the period lasted between January 2014 and December 2015. The most frequent clinical symptoms was the nasal discharge (100%), then signs like abscess formation and fever have been presented by 71.8% and 33.3% respectively. Most of clinical cases have been detected in age of six to twelve months (71.15%). Most of strangles-positive cases have recorded in winter season (60.03%) followed by spring (20%) and summer (18%). According to the biochemical results 75% of clinical cases caused by S. equi followed by S. zooepidemicus.S. equiinfection has been confirmed by the PCR technology through detection of Sod A gene.