National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181201Comparative assessment of the effect of Diminazene aceturate and Imidocarb dipropionate on haematology and serum biochemical parameters of apparently healthy Nigerian dogs.7581949910.21608/ejvs.2018.2693.1028ENJohnny OOlukunleDepartment of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta. Ogun State, Nigeria.0000-0002-0641-578XJournal Article20180123Diminazene aceturate <span lang="EN-GB">(DIAC)</span><span lang="EN"> and Imidocarb diproprionate (</span><span lang="EN-GB">IMD)</span><span lang="EN"> are drugs of choice in the management of some heamoprotozoan diseases. In this study we evaluated the comparative effects of the two drugs on the haematology and serum biochemical parameters of Nigerian local dogs. Fifteen (15) apparently healthy Nigerian dogs were used in this study. </span><span lang="EN-GB">Dogs were randomly divided into three equal groups. Group A received no treatment and served as</span><span lang="EN-GB">control. Groups B received intramuscular injection 3.5mg/kg DIAC and group C received 5.5 mg/kg of IMD via intramuscular injection. Blood samples were collected 24 hours after the administration of the drugs. through the cephalic vein from each dog for haematology and serum analysis. </span><span lang="EN">Packed cell volume (PCV), white blood cell (WBC), neutrophil, lymphocytes were counted. Serum aspartate aminotransferase (AST), alanine amino transferase (ALT), calcium, and chloride were measured. The overall results revealed that neither DIAC nor IMD treated groups caused any significant deviations from the untreated controls in all the above parameters assayed. In conclusion, both drugs are safe to use for treating heamoprotozoan diseases in the same dosage.</span>https://ejvs.journals.ekb.eg/article_9499_5ea2b8e58fc89c7b9bcb92646f08d983.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181231Evaluation of spermatogenic activity of Annona muricata methanolic bark extract in Albino rats8390990310.21608/ejvs.2018.2836.1029ENTunbosun AdemolaOladipoFederal College of Animal Heath and Production Technology, Moor Plantation, IbadanAdeniyi AdekunleAdebiyiDepartment of Veterinary Pathology, Federal University of Agriculture, Abeokuta.
NigeriaAbiodun SeunAdetomiwaDepartment of Veterinary Public Health and ReproductionOluwamayowa OmololaOladipoDepartment of Agricultural Engineering, Federal College of Agriculture, Ibadan.Olushola EmmanuelAdeleyeDepartment of Veterinary Physiology and Pharmacology, Federal University of Agriculture, Abeokuta. Nigeria.Johnny OOlukunleDepartment of Veterinary Physiology and Pharmacology, College of Veterinary Medicine, Federal University of Agriculture, Abeokuta. Ogun State, Nigeria.0000-0002-0641-578XJournal Article20180205Background: Annona muricata has been widely used by majority of people in the treatment of diabetes, pain, fever, cancer and many others diseases. Despite these medicinal values of this plant, it is important to study its effect on semen factors that determines reproductive health.
Materials and method:
Sixteen male rats weighing 150-200g were given <em>Annona muricata </em>methanolic bark extract (AMMBE) for fourteen days at the dose rate of 0 mg kg<sup>-1</sup>, 200 mgkg<sup>-1</sup>, 400 mgkg<sup>-1 </sup>and 800 mgkg<sup>-1 </sup>representing groups A, B, C and D respectively. The sperm cells were analyzed to determine the sperm counts, morphological appearances and survivability. Testes were also fixed in formalin for histopathology. The results obtained were analyzed using standard statistical package.
Results:
The results showed that the sperm liveability of rats treated with <em>Annona muricata</em> methanolic bark extract (AMMBE) were statistically significantly lower in groups B, C and D having mean values of 78.50±1.62, 86.50±3.11 and 68.00±1.96 respectively while the control group had 91.00±2.86, sperm motility was found to be highest in control group with 93.50±3.22 while other groups (B, C and D) had 82.25±2.20, 87.50±1.96 and 71.25±2.34 respectively. This finding showed strong correlation between the sperm motility and live-death ratio. In addition, there was reduction in the sperm concentration in a dose dependent manner, while the testicular histology revealed lesions that indicate potential for structural damages.
Conclusion:
It was concluded that due to the reductions in sperm motility, sperm concentration and live-death ratio, <em>Annona muricata </em>prolonged use may be detrimental.https://ejvs.journals.ekb.eg/article_9903_d783b02861dc806ee2a0edec9e5728aa.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181231Preparation of Biologically Active Recombinant Buffalo Follicle Stimulating Hormone (rbuFSH) from Buffalo pituitaries91102990610.21608/ejvs.2018.4189.1040ENAbdel Mohsen M. MohamedHammamDepartment of Animal Reproduction, Vet. Res. Div., National Research CentreAhmed NagyMohamedDept. Animal Reproduction, Vet. Res.Div., NRC0000-0002-0060-4328Ahmed MohamedKandeilDepartment of Water pollution National Research , Environ.Res.Div.,NRCJournal Article20180624Due to the immunogenic differences in the FSH-β subunits among animal species and the need for preparing specific gonadotropic hormones specific for buffaloes, the present work was aimed to prepare recombinant buffalo follicle stimulating hormone for using in improving the fertility of Egyptian buffaloes. Production of recombinant buffalo follicle stimulating hormone (rbuFSH) in <em>E.coli</em> by using recent biotechnological tools .Buffalo anterior pituitaries were collected from slaughtered buffaloes ,the buffalo common α-subunit and FSH β-subunit cDNAs was cloned and expressed to produce recombinant FSH from buffalo species <em>in vitro. </em>The RNAs extracted from buffalo pituitary glands will be reverse-transcribed and amplified by PCR and RT-PCR. The cDNAs corresponding to both subunits of buffalo hormones was cloned into the expression vector <em>E.coli</em> cells (DH5α) and pGEMT-Easy vector and transfected into pET 28a, pET 15b cells or pCR2.1-TOPO vector. Subcloning of the different subunits was carried out in pET vectors. Expression of genes was determined in the transfected cells by Northern and Western blot analysis. Recombinant buffalo FSH secreted in culture media was characterized by an <em>in vitro </em>bioassay. The recombinant products were injected to immature female rats compared to PMSG(standard) to stimulate ovarian growth and inducing of multiple follicles. The results revealed that the possibility of producing recombinant buff. FSH in vitro which are possessing efficient biological activity,stimulated the ovarian activity of immature female rats compared with standard PMSG hormone , which can be applied in buffalo-cows for improvement of reproductive efficiency .https://ejvs.journals.ekb.eg/article_9906_eeb347d042f6e288c469c45d53372ab2.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181230Iron Oxide Nanoparticles Versus Ferrous Sulfate In Treatment of Iron Deficiency Anemia In Rats1031091118610.21608/ejvs.2018.3855.1039ENMona AbdElzaherElshemyBenha University, Faculty of veterinary medicine, Clinical pathology departementJournal Article20180516Ferrous sulfate is the most commonly used drug for treatment of iron deficiency anemia, but it is badly absorbed and causes many unfavorable side effects. Nanotechnology is a way to decrease the side effects of drugs and to increase the drug bioavailability. So, this study is designed to investigate the effect of iron oxide nanoparticles in comparison to ferrous sulphate in the treatment of iron deficiency anemia in rats. Forty male albino rats were divided into two main groups: Control group (10 rats) and anemic group (30 rats) that received standard iron free basal diet for six weeks. Then the anemic group was subdivided into three groups (10 rats) in each group: anemic control group, ferrous sulfate group (received ferrous sulfate 0.4 mg/kg b.w/ 10 days) and iron oxide nanoparticles group (received iron oxide nanoparticles 0.4 mg/kg b.w/ 10 days) in the drinking water. Iron oxide nanoparticlescaused a significant increase in the level of red blood cells (8.80±0.05 10<sup>6</sup>/µL), hemoglobin (18.46±0.33 g/dL), hematocrit (46.66±0.23 %), mean corpuscular volume (MCV) (53.02±0.3 FL), mean corpuscular hemoglobin concentration (MCHC) (39.56±0.6 %), ferritin (447.6±9.02 µg/L), transferrin saturation (138.0±1.5), total iron binding capacity (TIBC) (145.00±1.15 mg/dL) and serum iron (276.33±2.07 mg/dL). Moreover, it decreased serum malondialdehyde (MDA) (31.85±0.34 nmol/g) and C-reactive protein (CRP) (312.66±1.7 mg/L) when compared to ferrous sulfate group and anemia control groups. These results revealed that iron oxide nanoparticles proved as an effective drug for the treatment of iron deficiency anemia in rats.https://ejvs.journals.ekb.eg/article_11186_bbf2956f75f04dfbf1f357efe5749e84.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181201Antidiabetic and Anti-hyperlipidemic Effects of Virgin Coconut Oil in Rats1111171118710.21608/ejvs.2018.3548.1036ENMona AbdElzaherElshemyBenha University, Faculty of veterinary medicine, Clinical pathology departementJournal Article20180414Virgin coconut oil is known to have beneficial health effects. Although it is rich in saturated fatty acids, these saturated fatty acids are easily absorbed and used as energy for metabolism and protects the body from disease. So, this study is designed to evaluate the protective effects of virgin coconut oil against alloxan induced diabetes.<br />
In this study, forty male Wistar rats were divided into four groups (10 rats per group), control group, virgin coconut oil (VCO) group, alloxan group and alloxan treated group. Alloxan was administered at 150 mg/kg body weight through a single intraperitoneal injection. After three days of induction of diabetes by alloxan, virgin coconut oil was administered orally at 10 ml/kg body weight, daily for four weeks.<br />
Results showed that alloxan caused diabetes as indicated by significant increase in the fasting blood glucose level (330±2.8 mg/dl) and oral glucose tolerance test with a significant reduction in serum insulin level (0.90±0.05 µiu/ml). Moreover, alloxan induced significant increase in total cholesterol (110±2.8 mg/dl), triglycerides (350±7.05 mg/dl) and LDL (33.60±0.98 mg/dl). Alloxan also induced renal dysfunction as indicated by a significant increase in serum creatinine (3.25±0.02 mg/dl) and urea (76.14±0.64 mg/dl). Alloxan caused significant elevations in ALT (108.2±1.06 U/L) and AST (118.9±0.76 U/L) with a significant decrease in serum albumin (2.36±0.17 g/dl) and total proteins (3.9±0.11 g/dl). However, coconut oil is effective in alleviation of alloxan induced disorders.<br />
It is concluded that coconut oil is very effective against deleterious hyperlipidemic, hyperglycemic and nephro-toxic effects of alloxan.https://ejvs.journals.ekb.eg/article_11187_3182cbb38737f4dfdc161b637d8c162f.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181201Studies on Pathogenicity of Local Newcastle Disease Genotype VIIj and Avian Influenza H9N2 Isolates to Commercial Vaccinated Male Layer Chickens1191331530610.21608/ejvs.2018.4958.1042ENMohamed MahrousAmerDepartment of Poultry Diseases.
Faculty of Veterinary Medicine.
Cairo University.
Postal Code: 12211 Giza, Egypt.0000-0001-8965-7698Journal Article20180829This study was carried out to compare pathogenicity of single and co-infection of Newcastle disease virus (NDV) genotype VIIj and Avian Influenza (AI) H9N2 virus in commercial male layer chicken vaccinated with live ND (Hitchner B1, La Sota) vaccines from different commercial producers in two vaccination schemes. NDV haemaglutination inhibition (HI (log2) mean titers in group received vaccines 1 (4.8 ± 0.8, 2.4 ± 1.2 and 3.9 ± 0.5) were lower than group given vaccine 2 (4.5 ± 1.2, 3.6 ± 1.1 and 4.0 ± 0.4) at 14, 21 and 29 days of age, respectively. Chicken group received ND vaccine 2 and inactivated H9 showed relatively higher HI titres (5.0 ± 1.5, 3.5 ± 1.4 and 4.5 ± 1.3). HI titres against H9N2 in group given H9N2 inactivated vaccine are 3.7 ± 1.6, 2.5 ± 1.1 and 5.1 ± 1.3 at 14, 21 and 29 days, respectively. Challenge with Newcastle disease virus (NDV) genotype VIIj at 33 days of age resulted in 100% mortality with severe signs as well as in non-vaccinated control sub-group challenged and 100% mortalities. Sub-groups (2a, 3a and 4a) vaccinated with ND vaccine 1, ND vaccine 2 and ND vaccine 2+ H9N2 showed signs of depression, off food and moderate respiratory signs with protection rates is 70, 70 and 75%, respectively. Control group challenged with H9N2 showed general signs with mild respiratory signs and 10% mortalities while, sub-groups given ND vaccine 2+ H9N2 and challenged with H9N2 showed 100% survival. Chicken sub-groups vaccinated with ND vaccine 1, ND vaccine 2 or ND vaccine 2+ H9N2 and challenged with NDV+ H9N2 (co-infection) showed signs form the 3rd s post infection (dpi) with moderate respiratory signs and protection rate of 50, 70 and 70%, respectively. Post-mortem lesions in Velogenic NDV (vNDV) challenged birds were septicemia, intestinal and respiratory lesion, while those challenged with both NDV and H9N2 showed more prominent lesions. The vaccinated groups showed unsatisfactory protection rates (50- 75%). Vaccine 2 showed higher protection and HI titers than vaccine 1. Simultaneous chicken challenge with H9N2 and vNDV pointed out that co-infection increased severity of clinical signs, mortality and gross lesions. Histological changes were reported in lung, intestine, and spleen of challenged non-vaccinated and vaccinated groups. The severity of tissue alteration was remarkably high in the non-vaccinated group and slightly mild tissue alteration in the vaccinated group. The histological changes ranged from severe congestion in blood vessels in tested organ with lymphoid depletion in spleen and hyperplasia of mucosal-associated lymphoid tissue in the intestine, with partial limited tissue change in the challenged vaccinated group.
The local NDV strain genotype VIIj is highly pathogenic to male layer commercial chickens and the inoculation of NDV with H9N2 at the same time didn›t increase its severity. Also, it was notable that commercial Hitchner B1 and La Sota vaccines conferred partial protection for experimentally used chickens against challenge this ND field isolate.https://ejvs.journals.ekb.eg/article_15306_932661874322b0b26d1ccbfd06fb0f7e.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181201Isolation and Identification of H9N2 Avian Influenza and Newcastle Disease Viruses co-Infections in Chicken1351461530710.21608/ejvs.2018.4963.1043ENMohamed MahrousAmerDepartment of Poultry Diseases.
Faculty of Veterinary Medicine.
Cairo University.
Postal Code: 12211 Giza, Egypt.0000-0001-8965-7698Journal Article20180829Co-infections with Newcastle disease (ND) and avian influenza (AI) highly pathogenic viruses causes high mortality and high economic losses. In this study, 322 samples were collected from 50 chicken
<span lang="EN">flocks suffering from respiratory symptoms, severe drop in egg production and mortalities from December, 2013 to December, 2014. Real time polymerase chain reaction (RT-PCR) was performed to detect the M protein of H9 gene of AI and F gene of velogenic ND virus (vNDV) co-infection. H9 PCR was 20 and 34 in cloacal and pharyngeal swabs with total percentage of 6.2 % and 10.5%, respectively. The H9 sequences showed low pathogenic RSSR/GLF motif. The phylogenetic tree showed that H9 sequences belong to the G1 lineage which clustered with 2015 -2016 Egyptian H9N2. Partial sequences of the Fusion gene revealed that the isolated NDV isolates belong to class II genotype VIIj. The cleavage site of the F protein showed the presence of a polybasic amino acid motif (112RRQKRF117) suggesting that our two isolates were vNDV strain. The molecularly identified H9 (1 isolate) and NDV Genotype VII (2 isolates) were named AI/CHICKEN/EGYPT/48Ob/NRC-CU/2014(H9), NDV/CHICKEN/EGYPT/48Ob/NRC-CU/2014, and NDV/CHICKEN/EGYPT/ 66Oc/NRC-CU/2014, and nucleotide sequences were submitted to GenBank and given accession numbers MG966504, MG966505, and MG966506, respectively</span><span lang="AR-YE">.</span><span lang="EN"> Conclusion: Natural mixed infections with AIV H9N2 and virulent NDV genotype VIIj viruses were detected in chicken flocks suffering from respiratory symptoms and mortalities in Egypt. </span>https://ejvs.journals.ekb.eg/article_15307_4805387efbe2bfef66d2220e2503ced3.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181201Study on β-haemolyticstreptococci Infection in Equines at Different Seasons and Ages1471541891510.21608/ejvs.2018.4986.1046ENOsamaMohamedinfectious disease department, college of veterinary medicine, cairo university, Cairo, EgyptDieaAbo ElhasanDepartment of Medicine and Infectious Diseases
Faculty of Veterinary Medicine
Cairo UniversityAmalElmollaDepartment of Medicine and Infectious Diseases,Faculty of Veterinary Medicine
Cairo University,Cairo, EgyptJournal Article20180901The present work was undertaken to study the incidence of β-haemolytic streptococci infection in equine. On clinical examination of a community of 524 Arabian foals and horses in Cairo – Egypt, a total of 164 animals were selected showing respiratory signs (31.30 %) of which 62 (11.83 %) showed respiratory signs and lymph nodes abscessation. Two hundred and twenty six swabs,164 nasal,31submaxillary lymph node pus and 31 retropharyngeal lymph node pus, were collected from 164 foals and horses showing respiratory signs and/ or abscessed lymph nodes for Streptococci isolation and PCR confirmation.<br /> A total of 150 isolates of Streptococci were recovered from 226 samples with sample-wise prevalence of (66.37%). Out of these 150 isolates, 124(28.67%) were identified as <em>Streptococcus equi</em>subsp<em>. equi</em>, 26(17.33%) as <em>S. equi</em>subsp.<em>zooepidemicus</em> and no S. dysgalactiae subsp. Equisimilis were identified.<br /> The incidence of <em>S. equi</em>subsp.<em>Equi</em> and <em>S. equi</em>subsp.<em>Zooepidemicus</em> infection among the total animal population, in the present study, was 11.83 and 4.96 % respectively. PCR technique showed high sensitivity and specificity for the detection of S. equi species in the examined samples.https://ejvs.journals.ekb.eg/article_18915_277c41cff32b02b7bc6e3e3345b36656.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181201Effect of Joint Inflammation on Piroxicam Pharmacokinetics in Rats1551651892010.21608/ejvs.2018.5311.1049ENAziza MahrousAmerDepartment of Pharmacology, Veterinay medicine collage, Cairo UniversityHanaaMabrokInstitute of Animal Health Research, DokkiJournal Article20181003Piroxicam is one of the nonsteroidal anti-inflammatory drugs of the oxicam class commonly used in both veterinary and human medicine. Anti-inflammatory and pharmacokinetics of piroxicam (20 mg/kg b. wt.) via intramuscular (i.m) injection were studied in normal and joint inflamed male rats. Injection of complete Freund’s adjuvant in the right hind rat’s paw resulted in biphasic edematous inflammatory thickness, immediate acute phase started at the 4th day followed by a chronic phase with marked edema at day 14 of injection. Treatment with piroxicam (20mg/kg b.wt.) for 28 day resulted in significant decrease in thickness of edema by 46.15, 57.40, 47.16, 53.57, 45.65 and 51.13% in days 4, 10,17,21,24 and 28 days, respectively.<br /> Treatment of arthritic rat with 20 mg/kg b. wt i.m piroxicam for 28 days showed a significant decrease in the arthritic index (2.00 ± 0.09 and 0.80±0.01) from day 10 to day 28 as compared with arthritic index in non-treated (2.80 ± 0.18 and 6.20 ± 0.15 ), respectively.<br /> Plasma samples were collected after 2,4,6,8,10,12,24 and 48 hours for analysis of plasma piroxicam concentration. The obtained data showed a non-significant increase in plasma piroxicam concentration in joint inflamed rats than that of normal rats. The calculated pharmacokinetic parameters revealed a short t0.5 absorption (t0.5ab) 2.10 ± 0.345 hrs and 1.75 ± 0.100 hrs, and prolonged elimination half-life time (t0,5el) 14.01 ± 0.730 and 20.61 ± 0.921 hrs in normal and joint inflamed rats, respectively. Slow elimination rate (Cl/F) (0.12 ± 0.003 and 0.08 ± 0.003 (mg/kg)/ (μg/ml)/h), t0.5el as well as prolonged MRT (23.24 ± 0.666 and 32.26 ± 1.261 hrs) in normal and joint inflamed rats, respectively.<br /> In conclusion: Piroxicam in dose of 20mg/kg/ b.wt via i.m has an anti-inflammatory effect in rat with joint inflammation and has non-significant higher and prolonged plasma concentration in inflamed joint rats than in normal.https://ejvs.journals.ekb.eg/article_18920_0bc33b5f295d51c05931eb49a4d25853.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181230Beneficial Effects of Non-Conventional Feedstuffs on Carcass Characteristics And Ileal Mucosal Morphology of Finisher Pigs1671772125510.21608/ejvs.2018.5040.1047ENJONAH NMUTHUIDepartment of Animal Science, Faculty of Agriculture, Egerton University, Njoro, KenyaJoseph WafulaMatofariDepartment of Food Science and Technology, Faculty of Agriculture, Egerton University, Njoro, KenyaAnthonyKingoriDepartment of Animal Science, Faculty of Agriculture, Egerton University, Njoro, KenyaChristianHülsebuschGerman Institute of Tropical and Subtropical Agriculture, University of Kassel, Germany.Journal Article20180905This study investigated differences in carcass characteristics and ileal mucosal morphology in pigs offered 4 isocaloric grower-finisher diets formulated using non-conventional feedstuffs from 99-180 days of age upon which barrows were slaughtered. The diets consisted of a basal maize-soybean meal diet, a 20% cold-pressed canola meal diet, 20% rice polishing diet and 20% rice bran diet. The 20% rice polishing diet resulted in a higher (p≤0.05) average daily gain compared to pigs on the basal diet. Rice polishing diet resulted in an increase in killing-out percent, 24.5% increase in loin eye area which implied better lean tissue deposition and a 19.3% decrease in villous height, and noted increases were significantly different (p ≤ 0.05). The 20% rice bran diet resulted in a higher (p≤0.05) average daily gain compared to pigs on the basal diet and larger loin eye size (p ≤ 0.05) implying better lean tissue deposition. Ileal villi were significantly longer (p ≤ 0.05) in in pigs fed rice bran diet and was attributed to higher dietary fibre. The 20% Canola meal diet increased (p ≤ 0.05) back-fat thickness and an increase (p ≤ 0.05) in ileal crypt depth. The study concludes that high NDF rice bran has reduces backfat and increases gut surface area thereby improving utilisation of dietary fibre. Low protein non-conventional feedstuff in finisher pigs had beneficial effects on ileal epithilia. Further studies on the role of dietary lipids and glycosinolates commonly present in Canola meal on changes in intestinal epithelial morphology should be done.https://ejvs.journals.ekb.eg/article_21255_9a2382f8faee049ab5f426964e5c09f1.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Veterinary Sciences1110-022249220181201The Seroprevalence Study of Reticuloendotheliosis Virus Infection in Chicken in Bangladesh1791862234210.21608/ejvs.2018.5856.1051ENMD ZULFEKARALIAnimal Health Research Division, Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka-1341, Bangladesh0000-0002-3971-8699Journal Article20181025We showed seroprevalence of <em>Reticuloendotheliosis virus</em> (REV) in poultry in selected areas of Bangladesh.Total 3,555 serum samples were collected between 2014 and 2016 from 10 districts, 6 types of chicken and 144 flocks, tested for the specific antibodies directed against REV by indirect Enzyme Linked Immunosorbent Assay (ELISA). The overall seroprevalence of REV in the chickens tested were 21.13% (751/3,555) and 73.61% (106/144) flocks were seropositive. According to district, Gazipur had the highest (49.50%) and Khulna had the lowest (9.09%) seropositive rates.Additionally, the prevalence rates were highest 38.21% during onset of laying period, age between 19 and 24 weeks. The positivity rates were almost same round the year like 22.96% in summer, 20.70% in rainy and 20.43% in winter seasons.Furthermore, the seropositive rates in broiler flocks, broiler breeder flocks, commercial layer flocks, layer breeder flocks, sonali flocks, and domestic chicken flocks were 6.86%, 42.15%, 25.84%, 33.59%, 9.40%, and 6.49%, respectively. Conclusion: These results indicate that REV infections are widespread ubiquitous in all types of chickens and may appear as a potential threat for the rapidly growing poultry industry in Bangladesh. This is the first seroprevalencestudy on REV infections in chicken in Bangladesh.More prevalence study, molecular characterization and vaccine production from local isolates are emergency requirements to combat REV. The quality verification of imported vaccine must be strongly adopted.https://ejvs.journals.ekb.eg/article_22342_3f1a238b52fac2ea9bdb767a3fab1bba.pdf