National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Some Udder Problems associated with productivity in Goats
1
9
EN
Youssef
fawzy
Ahmed
0000-0002-5408-6378
Prof.emeritus
yfahmed54@yahoo.com
Omima
H.
Ezzo
000-0002-1718-7807
Dept.Animal Reproduction & A.I
National research center
omimaezzo0@gmail.com
Sawsan
Mansour
0000-0002-0705-3784
Department of Animal production , National Research Centre, Dokki, Giza, Egypt
sawsan50man@yahoo.com
10.21608/ejvs.2019.13869.1087
The aim of this article was study the mastitis of a goat under farmer Egyptian conditions. This work was conducted on a private farm containing50baladigoats suffering from symptoms of mastitis. All environmental factors were recorded as housing condition, breeding, health problems, production and feeding. Morphological examination of the udder were carried out. Milk samples of infected goats, were collected for bacterial isolation and sensitivity test. Animals were treated with systemic and local intramammary broad spectrum antibiotic. The results indicated that, 35 cases (70 %) were responded to treatment, and showed normal reproductive and productive performances.15 cases (30 %) of chronic mastitis do not respond to treatment and breeding. Post-mortem examination of these cases revealed widespread diffused chronic mastitis throughout the affected udder. Tissue specimens of infected udder and supra mammary lymph node were fixed in 10% formalin for histopathological examination. Bacteriological examination revealed isolation of Staph aureus, Corynebacterium pyogenes, <em>Salmonella</em> spp., <em>E coli</em> in a single or mixed with other bacteria as <em>Pasteurella</em> spp. Fungus suspect <em>Asperigillus</em> spp. infection and yeast, suspect <em>Candida</em> spp. Pathological examination revealed chronic diffused suppurative and chronic granulomatous mycotic mastitis.<br /> In conclusion, mastitis is an important disease of goats and prevention of infection is the key for disease control. Good environ mental and hygienic housing of milking practices are a necessity to minimize the impact of this disease.
mastitis,Goat,Fertility and Productivity
https://ejvs.journals.ekb.eg/article_50311.html
https://ejvs.journals.ekb.eg/article_50311_020c6edfdfc1befe372fe3104538dede.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
PCR-SSCP and Sequencing Analysis For Studying Leptin Gene Polymorphism and Its Association with Reproductive Status of Egyptian Buffalo
11
21
EN
hazem
ahmed
eldebaky
0000-0003-0041-7546
animal reproduction, veterinary research division, national research centre
haldebaky@yahoo.com
Karima
Ghoneimy M.
0000-0002-0482-1207
prof Dr at National Research Center
karimamahmoud@yahoo.com
Khaled A.
Abd El-Razik
animal reproduction, vet. res. Division, national research center, Cairo, Egypt
khaled707@hotmail.com
Ahmed
Sosa
0000-0001-9726-2936
National Research Centre
ahmedsosa87@yahoo.com
Mohamed
kandil
Department of Theriogenology, Faculty of Veterinary Medicine, Benha University
kandiel75@hotmail.com
Youssef
fawzy
Ahmed
0000-0002-5408-6378
Prof.emeritus
yfahmed54@yahoo.com
10.21608/ejvs.2019.16438.1094
Leptin gene is one of the potential genes involves intricately in metabolism and growth of animals. Leptin has myriad effect on tissues and endocrine system that ultimately lead to the whole body energy metabolism and has major impact on performance and wellbeing of livestock species. Owing to have key biological features, leptin is one of themost significant candidate gene markers for Marker assisted selection (MAS) studies. This study was performed to identify leptin gene polymorphism and its relation to reproductive status in Egyptian buffaloes. Total of 150 buffaloes were subjected to ultrasound examination for evaluation of reproductive status. Blood samples were collected from all animals and were stored at -20°C till DNA isolation. Amplification of specific fragment 422bp (intron 2) of leptin gene was performed by polymorphism chain reaction (PCR). Single strand confirmation polymorphism (SSCP) was performed on PCR amplicons. Purified PCR amplicons of different electrophoresis patterns were sequenced in both directions using two primers. Sequences results were analyzed using NCBI BLASTn and BioEdit Sequence Alignment software.Two variants(AA and BB) of leptin gene were detected.Genotype AA represented by 64% in fertile buffalo, while its distribution among infertile one was 36%.Genotype BB, was equally distributed by 50% in both fertile and infertile animals. Sequence analysis of both normal and polymorphic one showed different singlenucleotide polymorphisms (SNPs) in leptin gene, but statistically these SNPs had no correlation with reproductive status (fertile or infertile) of examined buffalo.
Leptin gene,Buffalo,PCR-SSCP,Sequencing,Polymorphism,Reproduction
https://ejvs.journals.ekb.eg/article_51351.html
https://ejvs.journals.ekb.eg/article_51351_a0ec13c2e677cf32beb2ac7f74316467.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Determination of Synthetic Dyes in Various Food Samples of Iran’s Market and their Risk Assessment of Daily Intake
23
33
EN
Maryam
Zahedi
1 Research Center of Nutrition and Organic Products (R.C.N.O.P), Shahrekord Branch ,Islamic Azad University, Shahrekord ,Iran.
mahmood.bahmani@gmail.com
Amir
Shakerian
1 Research Center of Nutrition and Organic Products (R.C.N.O.P), Shahrekord Branch ,Islamic Azad University, Shahrekord ,Iran.
shakerianaa@ymail.com
Ebrahim
Rahimi
1 Research Center of Nutrition and Organic Products (R.C.N.O.P), Shahrekord Branch ,Islamic Azad University, Shahrekord ,Iran.
ebrahimrahi33@ymail.com
Reza
Sharafati Chaleshtori
2 Research Center for Biochemistry and Nutrition in Metabolic Disease, Kashan University of Medical Sciences, Kashan, Iran.
sheraf234@ymail.com
10.21608/ejvs.2019.16590.1095
THE AIM of this study was to measure the level of synthetic food dyes used in different foodstuffs of Iran’s markets via ion-pair high-performance liquid chromatography diode array detector (Ion-Pair HPLC-DAD). The chronic daily intake (CDI), target hazard quotient (THQ) and hazard index (HI) of synthetic dyes for Iranian population were calculated. Based on obtained results, from 74 foodstuff samples tested, only five synthetic dyes (Quinoline, Sunset yellow, Carmoisine, Allura Red and Brilliant Blue) were detected. Sunset yellow was the most commonly used synthetic colorant (45%). The highest concentration of synthetic colors were identified in fruit snacks (361 mgkg<sup>-1</sup>) followed by jelly product (161.9 mgkg<sup>-1</sup>), jelly powder (125.5 mgkg<sup>-1</sup>), ice products (104 mgL<sup>-1</sup>) and drinks (99.8 mgL<sup>-1</sup>). To the determine risk of exposure of Iranian consumers to five identified colors in all food product samples the CDI and THQ values were respectively lower than acceptable daily intake (ADI) recommended by WHO/FAO and one. Also, the HI values of all identified colors were lower than one, that did not represent a sever public health risk for synthetic color intake through assessed food samples in Iran<sup>’</sup>s market. Nevertheless, the results highlight the importance of a more attention monitoring of these dyes by the public and food health authorities Iran.
Synthetic dyes,Risk assessment,Iran’s market
https://ejvs.journals.ekb.eg/article_52629.html
https://ejvs.journals.ekb.eg/article_52629_32d02590ac1bbbc04fbf19b5244261bf.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Evaluation for Efficacy of Commercially Available Vaccines Against Challenge with Newcastle Disease Virus Genotype VII in Broilers
35
41
EN
Sameh
Abdel-Moez
Amer
0000-0002-6017-055X
Department of Poultry Diseases, Veterinary Research Division, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt..
drsamehnrc@hotmail.com
Asmaa
Mahmoud
Maatouq
Department of Poultry Diseases, Veterinary Research Division, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.
doc_asmaa@gmail.com
Hager
Magdy
Ahmed
Poultry and Rabbit disease Department, Veterinary Research Division, NRC
dr_gogo200810@yahoo.com
Eman
Ramadan
Hassan
Department of Poultry Diseases, Veterinary Research Division, National Research Centre, P.O. Code 12622, Dokki, Cairo, Egypt.
prof_emy@yahoo.com
10.21608/ejvs.2019.14003.1088
The study evaluated the protective efficacy of commercially available live and inactivated Newcastle Disease Virus (NDV) vaccines. Moreover, study also highlighted the implementation of genotypically-matched NDV vaccines to the currently circulated velogenic genotype VII strain in commercial broilers vaccination regimes in Egypt. Serum antibodies level was assessed by Haemagglutination Inhibition method .Efficacy of Newcastle Disease killed and live vaccine was also determined using challenge test. Group A immunized with live attenuated and inactivated NDV vaccines genotype VII homologous to challenge virus provided non-significant protection against mortality .It proved 20 % as compared to 15 % mortalities in group B immunized with live attenuated and inactivated NDV vaccines genotype II heterologous to challenge virus. Meanwhile, group Aredult proved a significant reduction in viral shedding by cloacal swabs in compared with group B ,7 days post-challenge. Final Results of the present work concluded that live and inactivated NDV genotype VII vaccines antigenically matched to currently epidemic NDV genotype VII providing better control on virus shedding if compared with different genotype II vaccines. However, both vaccines provided good protection against likely virulent challenge in commercial broilers.
vaccines,Newcastle,Disease Virus,Broilers,genotype VII
https://ejvs.journals.ekb.eg/article_53842.html
https://ejvs.journals.ekb.eg/article_53842_d9a7fdf5110d6f14aff825164536f316.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Chemical, Antioxidant, Total Phenolic and Flavonoid Components and Antimicrobial Effects of Different Species of Quinoa Seeds
43
54
EN
Zahra
Farajzadeh - First author
Ilam 1Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2Research Center of Nutrition and Organic Products, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Med Sci
mahmood.bahmani@gmail.com
Amir
Shakerian- Corresponding author
1Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2Research Center of Nutrition and Organic Products, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
shakeriana@ymail.com
Ebrahim
Rahimi
1Department of Food Hygiene, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2Research Center of Nutrition and Organic Products, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
ebrahimrah@ymail.com
Mahmoud
Bagheri
3Asistant Professor, Seed and Plant Improvement Institute (SPII), Agricultural Research, Education and Extension Organization, (AREEO), Karaj, Iran
baghmah@gmail.com
10.21608/ejvs.2019.17122.1098
Quinoa is considered as one of the newly emerged seed with high nutritional and biological aspects. The present research was done to study the biological activities of quinoa seeds. Quinoa seeds (cultivars; Giza1, Red Carina and Sajama) were obtained and their chemical properties were analyzed. Ethanoic extract was prepared and its total phenolic components (TPC), total flavonoid components (TFC), antioxidant and antimicrobial effects were studied. Saponin content was detected using the standard method. The highest contents of moisture, carbohydrate, protein, fat, fiber, ash and iron were found in cultivarSajama (7.23±0.61%), Giza1 (63.45±4.25%), Red Carina (18.11±1.62%), Sajama (10.19±0.98%), Red Carina (5.75±0.51%), Red Carina (5.98±0.46%) and cultivarGiza1 (26.80±2.51), respectively. The mean concentrations of saponin in cultivarGiza1 had the highest concentration of saponin (4.25±0.23 mg/g), while cultivarSajama had the lowest (1.84±0.12 mg/g). CultivarGiza1 had the highest TPC, TFC and antioxidant effects. CultivarGiza1 extract had the highest antibacterial effects. The minimum inhibitory concentrations of cultivarGiza1 (or Misr1) against <em>L. monocytogenes</em> and <em>E. coli</em> bacteria were 10 and 5 mg/ml, respectively, while those of <em>C. quinoa Sajama</em> against<em> L. monocytogenes</em> and <em>E. coli</em> were 80 and 40 mg/ml, respectively. The quinoa seeds are an appropriate alternative candidate of nutrients with high phenolic and flavonoid components and antimicrobial and antioxidant activities. High protein, carbohydrate, ash, fiber, iron, total phenol and flavonoid contents, low saponin concentration and considerable antibacterial and antioxidant effects of the quinoa seeds and particularly <em>C. quinoa Giza1</em> make them suitable as alternative functional sources of nutrients with high biological effects for human nutrition.
quinoa seed,chemical properties,antioxidant properties,Antimicrobial properties
https://ejvs.journals.ekb.eg/article_53914.html
https://ejvs.journals.ekb.eg/article_53914_43934bf9c45d1a392209a53903cdb515.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Morphometric Parameters and the Dynamics of Development of Quail (Coturnixcoturnix) Spleen
55
59
EN
Sudaba
Ismayilzada
Institut of Zoology National Academy of sciences of Azerbaijan.
Junior researcher, Applied Zoology Center
s-ismailzade@mail.ru
10.21608/ejvs.2019.16861.1096
In the present study <span lang="EN-GB">quails <em>Coturnixcoturnix</em><strong> (</strong></span><span lang="RU">Linnaeus, 1758) of 3 breeds- </span><span lang="EN-GB">Pharaoh, English White quail, Manchurian Golden</span><span lang="RU">were studied from Azerbaijan from June to September 2018. 120 quails were kept in 3 compartments of a large cage. </span><span lang="EN-GB">Materials were referred to in neonatal, juvenile sexual maturity, morphofunctional maturity periods.</span><br /> Up-to the end of researches the spleen grows intensively for 3 months. The decrease of all morphometric indicators of quails’ lifespan was recorded on the 120th day of quails. Comparing the recorded absolute weight of the spleen at 90 days it was decreased from 0.60g to 0.07g in Pharaoh breed, from 0.63g to 0.08g in the English White quails and from 0.57g to 0.06g in the Manchurian Golden quails.
Quails (Coturnix coturnix),spleen,Pharaoh,English White quail,Manchurian Golden
https://ejvs.journals.ekb.eg/article_55629.html
https://ejvs.journals.ekb.eg/article_55629_5c46cdcc9015021fc220e21589ae21ec.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Chromosomal Metaphase Spreads with G and NOR bandings of Fresh Water Teleost, Clarias batrachus
61
67
EN
Said I.
Shalaby
Dept. CAM, Medical Division, NRC, Cairo, Egypt
saidshalaby7@gmail.com
Neelima
Gupta
Vice Chancellor, Chhatrapati Shahu Ji Maharaj University, Kanpur, India
guptagrawal@gmail.com
D.K.
Gupta
Department of Zoology, Bareilly College, Bareilly, India
guptagrawal@rediffmail.com
V.K.
Verma
Department of Environmental Science, Future Institute of Engineering & Technology, Bareilly, India
neelamnegi@gmail.com
10.21608/ejvs.2019.17868.1104
Background,The detail structure of chromosomes depicted by their banding pattern is very useful in drawing homology and analogy in the structure of chromosome within and between the species.<br /> Results, In the present study, the diploid number of chromosomes was 50 in the fresh water teleost, <em>Clarias batrachus</em> in which 18 metacentric, 20 submetacentric and 12 telocentric chromosomes were observed. Structurally, the G bands revealed that transverse bands were visible after banding. These were due to the presence of heterochromatin and presence and absence of bases, these bands were observed at two pairs of submetacentric chromosomes. The NOR bands on the chromosomes were detected by silver staining in the two pairs of medium size telocentric chromosomes. It was believed that silver staining bound with the non-histone proteins of the transcriptionally active NOR region.<br /> Conclusion, Our results showed that basic information of chromosome number and morphology with G and NOR banding can be used as an important tool for cytogenetic data of fresh water teleost <em>Clarias batrachus</em>.
Chromosomal metaphase,G banding,NOR banding,Clarias batrachus
https://ejvs.journals.ekb.eg/article_57297.html
https://ejvs.journals.ekb.eg/article_57297_82b3fec6d571dec34d189ffa281b5dee.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
A New Modified Staining Technique for Demonstration of Prototheca Spp. Algae on Paraffin Sections First Record.
69
72
EN
Youssef
fawzy
Ahmed
0000-0002-5408-6378
Prof.emeritus
yfahmed54@yahoo.com
10.21608/ejvs.2019.18796.1115
The environmental <em>Prototheca</em> algae under certain condition are responsible for protothecosis infection in animal and human. The detail pathology and pathogenesis of this type of infection was unclear. Light microscopic diagnosis of <em>Prototheca</em> spp. algae is difficult with the use of routine histologic H&E stains. The specific stainsof fungusas periodic acid–Schiff (PAS) or Gomori Methenamine silver (GMS) were used and these stainsare not enough for examination of algae. A new modified staining method wasprepared. The main chemical constituents were Chromotrope2R, Aniline blueand phosphotungestic acid. The new stain gives very sharp affinity for staining of intra and extra cellular of <em>Prototheca</em> spp.microalgae and sporangium in different stages of maturation. The new stain will help the pathologist tostudy the pathology and pathogenesis of this type of pathogenic algae and considered the first specific stain for algae in tissue section.
Chromotrope 2R,Aniline-Blue,Prototheca algae,sporangium,sporangiospores
https://ejvs.journals.ekb.eg/article_58676.html
https://ejvs.journals.ekb.eg/article_58676_17eb9c1d2b16c9812ac19a99c01f5d4c.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Polioencephalomalacia Caused by Thiamine Deficiency in Sheep of Basrah Province, Iraq.
73
81
EN
kamal
Abd
Alsaad
https://orcid.org/00
Veterinary internal and preventive Medicine
College of Veterinary Medicine
University of Basrah, Iraq
kamalsad58@yahoo.com
10.21608/ejvs.2019.17509.1100
Polioencephalomalacia caused by thiamine deficiency in sheep of Barash province have been diagnosed. This current research was conducted on 44 local sheep breeds 1-3 years old and of both sexes reared in Basrah province. Ten clinically normal local sheep breeds were selected as controls. Diseased animals show various clinical manifestations, Such as Anorexia and head pressing, Muscular tremors. Moreover, diseased sheep show signs of opisthotonus and nystagmus, incoordination and ataxia, Furthermore, 41% of the diseased sheep show circling with lateral deviation of the head, recumbency and inability to stand. However, 18.1% of the diseased sheep exhibit blindness. Moreover, significant increase was detected in respiratory and heart rate of diseased animals in comparison with controls, but ruminal motility was decreased significantly in affected animals compared to controls. Additionally, considerable changes were detected in biochemical parameters of affected sheep in comparison with controls. The results of cross pathological features and histopathological examination of dying or slaughtered sheep show pale discoloration of the dorsal cerebral cortex, especially the occipital lobes, which might indicate a necrosis. However, gross lesions might also represent flattening of cerebral gyri, hemorrhages, necrosis to cavitations in the gray matter of the occipital and temporal regions. Further, histopathological examinations revealed gliosis as well to vacuolative changes of some neurons, Moreover, sections of the white matter of brain showed also gliosis with wallerian degeneration of some neuronal axons, Furthermore, sections were also indicated a severe vaculation of neuronal cell body and central chromatolysis of some neurons.
Thiamine deficiency,Polioencephalomalacia,sheep,Basrah,Iraq
https://ejvs.journals.ekb.eg/article_60041.html
https://ejvs.journals.ekb.eg/article_60041_c13f21a05fbf2a9e189038b6c3955fa9.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Preliminary Prevalence and Risk Factors of Mycobacterium bovis in Local and Imported Breeds of Cattle and Buffaloes in Mosul city, Iraq
83
88
EN
Osamah M.
Muwaffag
Aliraqi
0000-0001-8229-1232
University of Mosul / College of Veterinary Medicine
University of Mosul / College of Veterinary Medicine
tebavet@yahoo.com
Modrika
Al-Jammaly
0000-0001-9321-0768
University of Mosul / College of Veterinary Medicine
modrika1904@yahoo.com
Omer
AL-Hankawi
0000-0002-2220-8625
internal and preventive medicine,College of veterinar medicine,University of Mosul
khazaalvet79@yahoo.com
Maab
Ibrahim
Al-Farwachi
0000-0002-2288-6274
Department of internal and preventive medicine ,college of veterinary medicine,University of Mosul
maabalfrwche@yahoo.com
Mohammad
Osamah
Dahl
0000-0002-3595-1397
University of Mosul / College of Veterinary Medicine
University of Mosul / College of Veterinary Medicine
mohammad_osaamah@yahoo.com
10.21608/ejvs.2019.17753.1102
The objectives of the present study were to (i) investigate the prevalence of bovine tuberculosis in cattle and buffalo in Mosul, (ii) examine potential risk factors (source of animals, sex, and age), and (iii) indicate clinical signs associated to BTB in affected animals. A total of 196 animals (106 cattle and 90 buffalo) from different areas in Mosul. Serum antibodies againstbovine TB infection were examined by indirect ELISA. Prevalence was calculated, and potential risk factors and clinical signs associated to seropositive TB were examined using conditional logistic regression. Results indicated that the overall prevalence of bovine tuberculosis was 12.2% (18.9% in cattle, which was significantly higher than that (4.4%) in buffalo). The odds of seropositive for TB in imported cattle and female were 3 times higher, compared to local and male. There was no significant difference between age groups.Seropositive TB animals showed higher odds of having weakness and enlargement of lymph nodes, compared to those tested negative. All animals tested positive for TB exhibited intermittent hacking cough. In conclusion, tuberculosis is evident in bovine in Mosul, particularly in cattle, and the infection in imported cattle is a critical concern. Bovine TB should be considered in the differential diagnosis when the animals weak, exhibiting intermittent hacking cough, and having enlarged peripheral lymph nodes.
Bovine tuberculosis,ELISA,mycobacterium bovis
https://ejvs.journals.ekb.eg/article_60342.html
https://ejvs.journals.ekb.eg/article_60342_31ad05446b3e86a285bc240f0104ae6e.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Application of Phase- Contrast Microscope for Detection of Prototheca spp. Microalgae in Tissue Section-First record.
89
91
EN
Youssef
fawzy
Ahmed
0000-0002-5408-6378
Prof.emeritus
yfahmed54@yahoo.com
10.21608/ejvs.2019.18799.1116
Phase -contrast microscope was discovered in 1932 by the scientist Frits Zernike who won the Nobel Prize for Physics in 1953 for this scientific discovery. This technique is based on the fact that different parts of living cells have different densities and reflect the light with varying degrees and give different refractive parameters of the light. We used these phenomena to detect <em>Prototheca</em> spp. microalgae which are plant in origin and the wall contains sporopollenin which is biopolymers in nature and not found in animal tissue and this material is not in harmony with the contents of different animal cells. Therefore, it is possible to exploit this scientific phenomenon as a new method for the first time to identify <em>Prototheca</em> spp. microalgae in infected tissues.It has been possible to identify the infected tissue with <em>Prototheca </em>spp. microalgae with phase-contrast microscope as a simple rapid techniques, easy used for unstained slide. Also this technique may help the pathologist to study the life cycle and pathogenesis of this group of pathogen with the other specific standard methods for examination.
Phase- contrast microscope,Prototheca algae,microalgae
https://ejvs.journals.ekb.eg/article_60492.html
https://ejvs.journals.ekb.eg/article_60492_b67d2a71a853c36965e1184c7cf45ce3.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
An Anthelmintic Assessment of Balanites aegyptiaca Fruits on Some Multiple Drug Resistant Gastrointestinal Helminthes Affecting Sheep
93
103
EN
Hatem
A.
Shalaby
Department of Parasitology and Animal Diseases, National Research Centre, Giza, Egypt,
shalaby85@gmail.com
Noha
M,F
Hassan
0000-0001-9204-3240
Department of Parasitology and Animal Diseases, National Research Centre, Giza, Egypt,
nohamhassan555@yahoo.com
Soad
M.
Nasr
0000-0002-1538-8912
Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt.
soadnasr@yahoo.com
Tarek
Korany
Department of Parasitology and Animal Diseases, National Research Centre, Giza, Egypt,
tarek.korany@yahoo.com
Nadia
mohamed Talaat
Abu El Ezz
0000-0002-6228-610X
Depat. Parasitology, NRC,Cairo, Egypt
nadia_talaat60@yahoo.com
Hala A.
Abu zeina
0000-0001-7474-4596
Department of Parasitology and Animal Diseases, National Research Centre, Giza, Egypt
hala_zeina60@yahoo.com
10.21608/ejvs.2019.19195.1119
The goal of this study is to evaluate the effect of Balanites aegyptiaca methanolic extract fruits as an alternative sustainable source of anthelmintic drugs, on adult gastrointestinal multiple drug resistant helminthes and clarify how B. aegyptiaca fruits directly affect helminthes. To fulfill this study, three hundred and fifty sheep reared in a private farm, in Giza Governorate, had been subjected to clinical investigation. Of them, fifty five severely affected sheep had been isolated relying on their clinical history of multiple anthelmintic resistances, to be slaughtered. The post mortem examination revealed that the animals had a mixed infection with Haemonchus contortus, Moniezia expansa and Fasciola gigantica. Light and scanning electron microscopic studies were undertaken to evaluate changes in body wall of these adult worms following 24 h exposure to methanolic extract of B. aegyptiaca fruits utilizing concentrations of 10, 50, 100, 200 and 400 µg/ml. The main target organ that was highly affected was the body wall, whose damage was observed by light and scanning electron microscopic studies. These alterations occurred in particular sequences in response to Balanites extract concentration, initiated with swelling that was even disrupted, resulting in sloughing off cuticle or tegument, and finally exposure and disruption of basal lamina. This damage would induce disturbance for many of the physiological processes associated with the body wall, especially nutrition and immunoprotection. This plant may offer an opportunity for a new natural anthelmintic and an alternative source for the control of such infectious disease in sheep.
sheep,Haemonchus contortus,Moniezia expansa,Fasciola gigantica,Balanites aegyptiaca fruits
https://ejvs.journals.ekb.eg/article_62820.html
https://ejvs.journals.ekb.eg/article_62820_0b3a28d615d33db63d3ca9b6fa875643.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Pseudomonas aeuroginosa Biofilm and Antimicrobial Resistance
105
110
EN
farajolah
maleki
0000-0001-8887-8368
medicinal plant and biotechnology research center of Ilam university of medical sciences, Ilam, Iran
fmaleki88@yahoo.com
Saeed
Hemati
1.Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR Iran.
2.Student Research Comittee,Ilam University of Medical Sciences, Ilam, IR Iran.
saeedhemati0064@gmail.com
Zahra
Mahdavi
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR Iran.
zahramahdavi9165@gmail.com
10.21608/ejvs.2019.18523.1113
Introduction: It has been shown that biofilm formations are responsible for many human infections, especially in chronic infections. Pseudomonas aeruginosa (p.a) is an opportunistic human pathogen, its biofilm has serious negative effects on the health of patients with immune-suppresser e.g. cystic fibrosis individuals. p.a drug resistance, a worldwide concern is increasing and biofilm-forming potential is an increasing factor. Materials and Methods: One-hundred-fifty clinical P.a isolates were collected from Mustafa hospital in Ilam, then their resistance against several β-lactam antibiotics that high consumption in the medical prescription was assessment. Also, biofilm formation was tested by microdilution and microtiter plate methods, respectively. Results: There are abilities biofilm formations among the resistances isolates to the most tested antibiotics. But, we observed only a significant correlation between resistance to ceftazidime (P=0.003) and meropenem (P=0.002) with biofilm formation, both in resistance and sensitive isolates. Conclusion: Biofilm caused by Increasing drug resistance in the world, as β-lactam antibiotics, will be caused a creating complication P.a infection treatment. Moreover, biofilm can form antibiotic-resistant strains and increases many infections in the future. As a result, more studies for founding new drugs against biofilm formation mechanisms are necessary. <br /><br />
Pseudomonas aeruginosa,biofilm formation,β-lactam antibiotics
https://ejvs.journals.ekb.eg/article_64630.html
https://ejvs.journals.ekb.eg/article_64630_7cc62c08aadbbdb3951447754b009c87.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Prevalence Waterborne Infections after Earthquakes Considered as Serious Threat to Increasing Victims in Disaster-Affected Areas
111
117
EN
farajolah
maleki
0000-0001-8887-8368
biotechnology and medicinal plants research center of Ilam university of medical sciences, Ilam, Iran
fmaleki88@yahoo.com
Saeed
Hemati
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, IR Iran.
saeedhemati0064@gmail.com
Rashin
Pourashraf
Lecturer, Health Center, Isfahan University of Medical Sciences, Isfahan, Iran
pourashrafrashin@yahoo.com
10.21608/ejvs.2019.18629.1114
The disasters are competent for beginning the prevalence of waterborne infections. The earthquakes are the most important disaster worldwide. Studies have reported waterborne pathogens can causing about thirty-eight types of infections after each earthquake. Also, waterborne pathogens can cause a higher fatality than an earthquake. Therefore, a strategy for prevention from infections that may occur for people is important after each earthquake. Waterborne infections were sometimes causing two folds fatalities than the earthquake. Disasters are the most important complications in developing countries for the World Health Organization (WHO). Notably, occurrences of infectious diseases after natural disasters commonly occur, especially in seismic regions. Iran is located in the Alpine-Himalayan seismic belt and considered a seismic country. The planning of an infectious disease strategy is necessary for decreasing health problems. Therefore, our aims of the present study were to introduce important bacterial waterborne infectious agents that prevalence after the previous earthquakes and explained several important relevant prevention strategies.
Earthquake,Waterborne infections,Serious Threat
https://ejvs.journals.ekb.eg/article_64975.html
https://ejvs.journals.ekb.eg/article_64975_b73452cf4533331c868eacda4ca1cacf.pdf
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
51
1
2020
04
01
Therapeutic Effect of Balanites Aegyptiaca Fruit’s Ethanol Extract in Egyptian Baladi Goats Experimentally Infected with Haemonchus Contortus: Blood Serum Biochemical, Oxidative Stress Markers and Pathological Studies
119
136
EN
Ezatullah
Jaheed
Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
jaheedezatullah@yahoo.com
Amira
H.
Mohamed
Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt.
damira115@yahoo.com
Soad
M.
Nasr
0000-0002-1538-8912
Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt.
soadnasr@yahoo.com
Khaled
M. A.
Mahran
Department of Clinical Pathology, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt.
k.mahran@cu.edu.eg
Faten
F.
Mohammed
Department of Pathology, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt.
fatenfathy21@yahoo.com
Doaa
Sedky
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, 33 Bohouth Street, Dokki, P.O. Box 12622, Giza, Egypt.
doaa_sedky@yahoo.com
Hala
A.A.
Abou-Zeina
0000-0001-7474-4596
Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Giza, Egypt
hala_zeina60@yahoo.com
10.21608/ejvs.2019.20398.1140
The therapeutic effect of Balanites aegyptiaca fruit’s ethanolic extract (BAF-EE) in goats experimentally infected with Haemonchus contortus (H. contortus) was evaluated. Serum biochemical parameters, tissue oxidative stress status and pathological changes were investigated. Twelve goats were divided into 4 equal groups, G1 (infected-untreated), G2 (infected-BAF-EE treated), G3 (infected-albendazole treated) and G4 (uninfected-BAF-EE treated). Infection was conducted at 0-time, whereas treatment with BAF-EE and albendazole was given in the 5th week post infection (PI). Blood samples were drawn on 0-time, 3rd week PI and then biweekly till the 9th week for serum biochemical analyses. At the end of experiment, all animals were slaughtered to estimate oxidative stress markers and histopathological alterations in the abomasal and hepatic tissues. Serum biochemistry of infected animals showed significant depression of total serum proteins, albumin, albumin/globulin ratio, total antioxidant capacity and calcium levels. The activity of AST, ALT and γGT and the cholesterol, urea and creatinine levels were significantly elevated in the serum of infected animals. Glutathione peroxidase values decreased significantly in tissue homogenate of G1 and G3 goats than those in G2 and G4 goats. Malondialdehyde followed a reverse course. Abomasa of infected animals in G1 revealed inflammation, necrosis, petechial hemorrhages and cellular infiltration. Liver showed bile duct hyperplasia, degeneration of hepatocytes and cell infiltration. Intensity of alterations were lower in infected–treated animals. In conclusion, BAF-EE has hepatoprotective effect and can improve serum biochemical alterations and oxidative stress status in goats infected with H. contortus, justifying its use as a safe natural anthelmintic agent.
Haemonchus contortus,goats,Blood serum biochemistry,Pathology,Balanites aegyptiaca
https://ejvs.journals.ekb.eg/article_66629.html
https://ejvs.journals.ekb.eg/article_66629_20172f501af26ff0ac0e8aeb49166e0a.pdf