eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-07-01
47
1
1
12
10.21608/ejvs.2016.1077
1077
Original Article
Investigation of the Booroola Gene in Egyptian Ewes with Different Reproductive Status Using PCR- RFLP
Hiam N. Ahmed
1
Karima Ghoneimy M.
karimamahmoud@yahoo.com
2
M.M. Kandiel
3
Nermeen A. Helmy
4
S.S. Ibrahim
5
M.F. Nawito
6
O.E. Othman
7
Department of Animal Reproduction & A.I., National Research Centre,
prof. Dr. at National Research Centre Department of Animal Reproduction & A.I.
Department of Theriogenology, Faculty of Veterinary Medicine, Benha University
Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University
Department of Animal Reproduction & A.I., National Research Centre
Department of Cell Biology, National Research Centre, Cairo, Egypt.
OVULATION CAN BE regulated by group of genes, termed as fecundity (Fec) genes. The aim of this study was to identify genetic polymorphism in Booroola (FecB) gene in Barki, Rahmani, and Ossimi sheep breeds with different physiological status diagnosed by ultrasound. Accordingly, animals were early classified into three groups: carrying single fetus, twin fetuses and non-pregnant by ultrasonographic examination. Demonstration of fetal number was available as early as Day 35-40 post-mating transrectally, and trans-abdominally then after. The fetal viability could be checked through heart examination with M-Mode. Genomic DNA was extracted from blood samples of total number of sheep and two primers were used to amplify 190 and 140 bp fragments of FecB gene. The amplified fragments were digested using AvaII restriction enzyme. All sheep groups were non carriers for the FecB mutation and gave a 190 bp band (++) and 140 bp band (Fec++) for primer 1 and 2, respectively.In conclusion,no genetic polymorphism was detected in the three Egyptian sheep breeds in relation to pregnancy with single or twin fetuses. The study could be continue to search for other major genes.
https://ejvs.journals.ekb.eg/article_1077_24c680b2938207c2ae84b8a3f10dec3b.pdf
sheep
Booroola (FecB) gene
Genotyping
Ultrasound
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-07-01
47
1
13
26
10.21608/ejvs.2016.1079
1079
Original Article
Semen Characteristics and Genotyping of Pituitary-Specific Transcription Factor Gene in Buffalo Using PCR-RFLP
M.H. Hasanain*,
1
Karima Mahmoud*,
2
A.A. El-Menoufy**,
3
A.M. Sakr ***,
4
Y.F. Ahmed*
5
O.E. Othman****
6
*Department of Animal Reproduction & A.I., Veterinary Research Division, National Research Centre
*Department of Animal Reproduction & A.I., Veterinary Research Division, National Research Centre
**Department of Theriogenology, Faculty of Veterinary Medicine, Cairo University,
***Animal Production Research Institute, Agriculture Research Centre
*Department of Animal Reproduction & A.I., Veterinary Research Division, National Research Centre,
****Cell Biology Department, National Research Centre, Cairo, Egypt.
THIS STUDY aimed to evaluate the sperm characteristics in buffalo bulls and to screen the genetic polymorphisms in PIT-1 gene as bases for selection of bulls with good breeding value. The study was performed on 60 buffalo bulls aged 2-8 years. The animals were divided into three groups according to the age. The first group were between 2 to <3 years (n= 35). The second group were between 3 to <5 years (n=14). The third group were between 5 to <8 years (n=11).Three semen collections were obtained from each animal at 15-day intervals and evaluated for volume, individual motility, live sperm and chromatin integrity %. The semen samples were stored at 20 °C until DNA extraction then polymerase chain reaction (PCR) and DNA amplification were carried out.Restriction fragment length polymorphism (RFLP) was used for genotyping of pituitary-specific transcription factor gene using HinfI-RFLP. The results showed that, ejaculate volume, individual motility and live sperm % were significantly lower in old than adult and young buffalo bulls. While chromatin damage percentage had no significant difference among groups. All buffalo bulls were genotyped as BB with the predominance of B allele where PCR 451 bp fragment was digested into two fragments 244 and 207 bp.
It may be concluded that age had adverse effect on semen quality. Monomorphic pattern of the amplicon 451 bp in PIT-1HinfI locus in exon 6 was fixed in Egyptian buffalo with the predominance of B allele and BB genotype in a high frequency (100%).
https://ejvs.journals.ekb.eg/article_1079_81cd0980280ae05a6dccbbf81c1dbeed.pdf
Buffalo
Semen evaluation
PIT-1(POU1F1) gene
Polymorphism
RFLP
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-07-01
47
1
27
39
10.21608/ejvs.2016.1080
1080
Original Article
Influence of Epidermal Growth Factor with Cysteamine on in -Vitro Buffalo Embryo Development
Al-shimaa El-Naby
dvet_shima@hotmail.co.uk
1
Karima Ghoneimy M.
karimamahmoud@yahoo.com
2
Tarek Scholkamy
samahtarek@hotmail.com
3
Youssef Youssef
yfahmed@yahoo.com
4
Gamal Sosa
gamal_sosa@yahoo.com
5
Mahmoud Abou El-Roos
abouelroosmahmoud@yahoo.com
6
Faculty of Veterinary Medicine, Benha University
prof Dr at National Research Center
Animal Reproduction Research Institute
National Research Center, Egypt
Faculty of Veterinary Medicine, Benha University, Egypt.
Faculty of Veterinary Medicine, Benha University
FOR IMPROVING embryo development in buffalo, two experiments were conducted. The first one was carried out to evaluate the different concentrations (0, 5, 25, 50 ng/ml) of epidermal growth factors (EGF) on developmental competence of buffalo oocytes. The selected oocytes were cultured in the four concentrations of EGF. The embryo cleavage rate was significantly higher in oocytes exposed to 5, 25, 50 ng/ml EGF than control. There were no significant difference in four groups (0, 5, 25, 50 ng/ml EGF) in the rate of morula. But, better cleavage and blastocyst rates were observed at 5 ng/ml EGF. In the second experiment, the additive effect of 5 ng/ml EGF with 50 µM cysteamine on maturation and embryo development was studied. Oocytes were collected, matured and cultured in three groups. In the first group, media supplemented with 5 ng/ml EGF + 50 µM cysteamine combination. In the second group, media supplemented with EGF. The third group was supplemented with cysteamine. There was a significant increase in cleavage rate in combination group than EGF (P< 0.05) and cysteamine (P< 0.01) groups. But there was no significant difference in cleavage rate between EGF and cysteamine. The morula percentage was nearly similar in the three groups. But blastocyst rate was significantly (P<0.05) higher in combination group than cysteamine. Thus better cleavage and blastocyst rate were observed at combination group. It is concluded that, the addition of 5 ng/ml EGF in buffalo oocytes during in vitro culture was the best concentration for embryo developmental competence. Higher cleavage and blastocyst rate were achieved by combination of epidermal growth factor and antioxidant.
https://ejvs.journals.ekb.eg/article_1080_29f9434d5b218f236b3abacdf3be056e.pdf
Buffalo oocytes
EGF
Cysteamine
Embryo development
culture media
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-07-01
47
1
41
50
10.21608/ejvs.2016.1081
1081
Original Article
Single Nucleotide Polymorphisms of GnRHR Gene and Its Relationship with Reproductive Performance in Egyptian Buffaloes
Ahmed Sosa
ahmedsosa87@yahoo.com
1
Karima Ghoneimy M.
karimamahmoud@yahoo.com
2
Hazem Eldebaky
haldebaky@yahoo.com
3
Mohamed Kandiel
kandiel175@hotmail.com
4
Mahmoud Abou El-Roos
abouelroosmahmoud@yahoo.com
5
Mahmoud F. Nawito
prof_nawito@hotmail.com
6
National Research Centre
prof Dr at National Research Center
National Research Centre
Faculty of Veterinary Medicine, Benha University,
Faculty of Veterinary Medicine, Benha University
null
THIS WORK aimed to search for single nucleotide polymorphisms of gonadotropin releasing hormone (GnRHR) gene in Egyptian buffalo which involved in endocrine control of fertility. An ultrasonographic examinations of anestrum and repeat breeders’ buffaloes wereperformed prior to blood sampling through a transrectal ultrasonography. DNA was extracted from blood and the PCR and single-strand conformation polymorphism (SSCP) techniqueswereadopted to study of GnRHR gene polymorphism. The PCR amplified a fragment with 240-bp in size and the SSCP results showed that there isagenetic polymorphism with three different patterns (AA, BB and CC) in Egyptian buffalo. The CC genotype was associated with smooth inactive ovary,while BB genotype was associated with fertility in buffalo. Multiple sequence alignment of the three patterns sequences revealed that GnRHR had 5 single nucleotide polymorphisms including one nucleotide insertion, one nucleotide deletion and 3 nucleotide substitutions. Insertion was at position 4 in pattern III. The deletion was at position 189 in pattern III, while the 3 nucleotide substitutions were at positions 204 (T/G), 206 (T/A) and 207 (A/T). In conclusion, GnRHR gene could be used as a candidate marker for fertility in Egyptian buffaloes with its mutation is relatedto ovarian inactivity.
https://ejvs.journals.ekb.eg/article_1081_cad35ba9d40485325d467adcf4b91401.pdf
Buffalo
GnRHR Gene
Nucleotide sequences
SSCP analysis
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-07-01
47
1
83
110
10.21608/ejvs.2016.1082
1082
Original Article
Evaluation of the Protective Effect of Ethanolic Extract of Plantago major Plant Against CCl4 Induced Genotoxicity and Biochemical Disturbance in Rat
Zakaria Nofal
zakaria1948@yahoo.com
1
Assam El-Maghraby
elmaghrabyaf@yahoo.com
2
Mohamed Gad
mohamedrabie@yahoo.com
3
Ayman Farghaly
ayman.a.farghaly@gmail.com
4
Essam Ahmed
essamma2010@gmail.com
5
Biochemstry Department, Faculty of Agriculture, Al-Azhar University
Biochemstry Department, Faculty of Agriculture, Al-Azhar University
Food Technology Research Institute, Agricultural Research Centre
Genetics and Cytology Dep . NRC
Orimental and Medicine Plants Department, Horticulture Research Institute, Agricultural Research Centre
This study was carried out through 2010-2014, seasons on on …fresh herbs of Plantago major plant, which was collected from a private nurseries of El Qanater el Khayreyya, Al- Qaluobia Governorate, Egypt. The aim of this study was to investigate the protective potential of ethanolic crud extract of Plantago major against oxidative stress induced by carbon tetrachloride (CCl4) in different tissues in rats. Our results observed that CCl4 exhibited a significant increase in the levels of serum glucose and lipid profile i.e. total cholesterol, triglyceride and low density lipoprotein cholesterol (LDL) with a significant decrease in the level of high density lipoprotein cholesterol (HDL). Also, CCl4 increase liver function including serum aminotransferases (AST and ALT), alkaline phosphatase (ALPh), total protein, albumin (ALB) and total bilirubin activities and kidney function including the levels of serum urea, uric acid and creatinine amounts. Treatment with ethanolic P. major extract at 100mg/kg b.wt before, with and after treatment with CCl4 significantly prevented all of these typically observed changes . Also, P. major extract statistically significant (P<0.01) inhibit DNA damage induced by CCl4 in bone marrow and sperms of rats. Our findings indicate that P. major has a significant protective effect against CCl4 induced genotoxicity and biochemical changes in rat, which may be due to its antioxidant properties.
https://ejvs.journals.ekb.eg/article_1082_dfe8d81c0c69949181bcac9eff1a18a0.pdf
Plantago major herb
Carbon tetrachloride
Chromosomal abnormality
Biochemical analysis
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-07-01
47
1
63
81
10.21608/ejvs.2016.1084
1084
Original Article
Disease causing organisms in Procambarus clarkii and Gambusia affinis with emphasis on their role in biomonitoring of aquatic pollution
Waleed Soliman
waleed0809@yahoo.com
1
Wafaa Abbas
wtabbas2005@yahoo.com
2
Taghreed Ibrahim
mkarema27@hotmail.com
3
Amany Kenawy
amanyk70@yahoo.com
4
Mamdouh Elgendy
mamdouhyousif@yahoo.com
5
Assistant professor Hydrobiology Department, Veterinary Research Division, National Research Center
Assistant professor in hydrobiology department, Veterinary Science Devision, National Research Center.
Assistant professor, Department of Hydrobiology, Veterinary Research Division, National Research Centre
Professor of pathology, Department of Hydrobiology, Veterinary Research Division, National Research Centre
Researcher of microbiology, Department of Hydrobiology, Veterinary Research Division, National Research Centre
The health status of red swamp crayfish, Procambarus clarkii and mosquito fish, Gambusia affinis collected from Elmansoria canal, Giza, Egypt was investigated. The canal is known to receive lofty loads of pollutants from diverse anthropogenic sources. 113 bacterial isolates were obtained from the investigated fish specimens. Isolates were phenotypically identified as; Aeromonas hydrophila 26.54%, Vibrio parahaemolyticus 21.23%, Pseudomonas fluorescens 14.15%, E. coli 10.61%, Citrobacter spp. 7.96%, Enterobacter sp. 8.84%, Staphylococcus sp. 4.42% and Micrococcus sp. 6.19%. High gill infestations with Centrocestus sp encysted metacercariae were noticed in mosquito fish. No parasitic infestations were recorded in crayfish. Challenge experiment confirmed the pathogenicity of Aeromonas hydrophila isolates. The water analysis revealed high heavy metals levels with values; Ni 0.71, Pb 0.34, and Cd 0.2 ppm while Zn and Cu were in normal values. Metals analysis in crayfish and mosquito fish tissues denoted bioaccumulation. Crayfish muscles showed; Ni >Zn >Cu >Pb >Cd while their levels in mosquito fish demonstrated; Ni >Zn> Pb> Cd> Cu. Proliferative, degenerative and necrotic alterations were evident in histological sections. Results suggest that both crayfish and mosquito fish can serve as carriers for some fish disease pathogenic agents and a convenient tool for biomonitoring aquatic pollution.
https://ejvs.journals.ekb.eg/article_1084_3cc8e7466ed58210a222f47407339195.pdf
Bacteria
Parasites
pollution
Crayfish
Mosquito fish
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Veterinary Sciences
1110-0222
2357-089X
2016-07-01
47
1
51
61
10.21608/ejvs.2016.1162
1162
Original Article
The Effect of Freeze Drying Using Different Media and Storage Temperatures on Some Parameters of Buffalo Bull Spermatozoa
mohamed shahba
shahbavet@yahoo.com
1
Reda El-Sheshtawy
rielsheshtawy@gmail.com
2
Abdel-Salam El-Azab
elazab1949@fvtm.bu.edu.eg
3
Alaa Abdel-Ghaffar
abdel_ghafar@yahoo.com
4
Maha Ziada
maha_ziada@outlook.com
5
Adel Zaky
adel_hazky@hotmail.com
6
National research centre
Animal Reproduction and AI Department, Veterinary Division, National Research Centre, Dokki
Faculty of Veterinary Medicine, Benha University
Faculty of Veterinary Medicine, Benha University
Animal Reproduction Research Institute, Al-Haram
Animal Reproduction Research Institute, Al-Haram
THE PRESENT study was designed to display the effect of freeze- …..drying (lyophilization) technique using different freeze-drying media and storage temperatures on some parameters of buffalo bull spermatozoa. The semen samples were collected once weekly from five mature buffalo bulls maintained in Animal Reproduction Research Institute, Ministry of Agriculture, Al- Haram, Giza, Egypt. Samples were allocated into two portions; the first one was cryopreserved in Tris-Fructose-Egg yolk-Glycerol, while the second portion was immediately freeze-dried. These two portions were exposed to the same technical procedures. The freeze-dryer set was programmed at -55°C and 0.001 mbar pressure. The media tested were: EGTA solution, EDTA solution, TCM199 with Hanks salts enriched with 10% fetal calf serum (FCS) and TCM199 with Hanks salts enriched with 10% FCS and 0.2 M trehalose. The storage temperatures experienced were (4, - 20, - 80 ºC). The efficiency of each medium and storage temperature on some sperm parameters was explored. Our results revealed no motility either in raw or frozen-thawed spermatozoa after freeze-drying. In case of raw and frozen-thawed semen; with no respect to storage temperatures, the best freeze-dried sperm parameters were observed in TCM-Trehalose medium, while the best storage temperature was (-80°C) followed by (-20°C) with no respect to the type of freeze-drying media. From the present study, it was concluded that the freeze-drying medium containing trehalose could preserve efficiently components of buffalo bull spermatozoa, especially the acrosome, while the storage temperature (-80°C) and (- 20°C) were the best for storage of freeze-dried buffalo bull spermatozoa.
https://ejvs.journals.ekb.eg/article_1162_8d65abb251687909a9cc3f59072f5192.pdf
Buffalo
freeze-drying
EGTA
EDTA
trehalose
acrosome